Cloning and expression of functional reteplase in Escherichia coli top10. Avicenna J Med Biotechnol. 2013;5(3):168-175.Khodabakhsh F, Dehghani Z, Zia MF, Rabbani M, Mir Mohammad Sadeghi H. Cloning and expression of functional reteplase in Escherichia coli top10. Avicenna J Med Biotechnol. ...
coli TOP10 cells to generate the recombinant plasmid designated pETDN. Similarly, we obtained other recombinant plasmids, including pETDNT, pETDNTO, pETDL, pETDS, and pETDC. Fermentation For shake flask fermentation, a single colony was carefully chosen and introduced into 50 mL of LB culture ...
coli strains tested, TOP10 strain grown on agar plates induces a homeotropic orientation of LCs whereas DH5α and JM109 strains do not. This results in a clear distinction in the optical appearance of LCs as either uniformly dark or bright under polarised light. The LC-based method provides...
At the chromatin level, methylated CpG dinucleotides are R.Msp1 resistant compared with nonmethylated counterparts. The DNA of two E.coli strains was analyzed following transformation- with bacterial cytosine-5-methyltransferase gene M.Msp1 in mammalian transfection vector pcDNA3. Expression of the M...
ESCHERICHIA COLI (K-12 ST 10 MG,E2864,Invitrogen市场价: 询价 /套 在线询价 优惠: 请询价 订货号 : 5WJ7165 品牌型号 : Invitrogen E2864 货期 : 询货期 最小订货量 : 1套 + - 加入购物车 Invitrogen 联系我们 400-920-3909 微信扫一扫,手机采购 ...
coli Top10 harboring the SuperCosI empty vector control were designated APE− (Fig. 2A, B). As expected, most mutants in predicted core biosynthetic genes (represented in shades of blue in Fig. 2A) are APE−. Notably, deletion of apeA, the first gene in the BGC, retains wild-type ...
coli TOP10 (Invitrogen, Carlsbad, CA, USA) unless otherwise stated. Com- mercial Luria–Bertani (LB) was used as the medium for cloning unless otherwise stated. Kanamycin (30 mg ml À 1) was added to the culture medium for antibiotic selection where appropriate. P. aeruginosa In7, a ...
coli Top10 pcs+ was tested at first, and then beta-lactamase activity in periplasm was examined. Finally Western blot was used to detect the amount of beta-lactamase in both bacterial periplasm and cytoplasm. RESULTS: Antibiotic tests showed that high concentrations of ampicillin inhibited the ...
coli TOP10F' strain used in our laboratory for molecular cloning, but not in any of its precursor strains, including TOP10. Furthermore, bacteria cultured from the mouth of the laboratory worker who performed cDNA RDA were also positive for ILG1.In the course of our studies using cDNA RDA...
大肠杆菌(Escherichia coli,E. coli)在生物制药工业中生产重组蛋白方面发挥了重要作用,它是第一个用于生产生物药物的表达载体,其发展促使了1982年首个生物技术产品Humulin®(用于治疗糖尿病的人胰岛素)的诞生。E.coli具有生长快速、基因操作方便、重组蛋白合成速度快等优点,也存在一些固有障碍,如密码子使用偏好、缺乏翻...