BL21仅表达E.coliRNA聚合酶,适合依赖于E.coliRNA聚合酶的质粒载体,如pGEX、pMAL等;存在泄漏表达(Leaky expression),即在没有诱导剂的情况下也有一定程度的蛋白表达。 1.1 大肠杆菌BL21(DE3) 被λ噬菌体DE3溶原化获得的BL21衍生菌株,DE3区域中的lacUV5启动子控制T7 RNA聚合酶基因,通过异丙基硫代半乳糖苷(Isopropyl...
coli BL21(DE3) 螖nanA 螖nag 螖poxB as host, a significant 16.5% yield improvement was obtained. Furthermore, precursor (pyruvate) feeding resulted in 3.2% yield improvement, reaching 66.8% molar biotransformation. The result highlights the importance of host optimization, ...
产品名称 Escherichia coli BL21 Star(DE3)plysS 商品货号 B80883 基因型 F-ompT hsdSB (rB-mB-) gal dcm rne131 (DE3)pLysS (CamR) 基本信息 BL21 Star (DE3)pLysS菌株来源于BL21 (DE3),含有rne131突变(RNaseE基因突变),RNaseE基因的突变降低了内源RNase的积累,增强了菌株细胞内mRNA的稳定性,从而提高了...
产品名称Escherichia coli BL21(DE3) 商品货号B98190 基因型F- ompT hsdSB (rB- mB- ) gal dcm (DE3) 基本信息本菌株适用于T7启动子(T7 promoter)调控目的基因表达的pET系列等原核表达质粒。 BL21缺失细胞质中的Lon蛋白酶(Lon protease)和胞外膜上的OmpT蛋白酶(OmpT protease),能有效避免重组表达蛋白的降解...
BL21(DE3)大肠杆菌,Escherichia coli BL21(DE3),SHBCC D24923,BL21(DE3) 公司简介 上海瑞楚生物科技有限公司(www.ruichubio.com)是一家以科技为驱动力,以市场为导向的科技型企业,公司产品依托江苏工厂(瑞楚生物科技(江苏)有限公司)负责生产。公司目前拥有自我品牌"瑞楚生物REBIO",其业务核心是利用AI驱动自动化设备...
www.nature.com/scientificreports OPEN Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains received: 25 October 2016 accepted: 20 February 2017 Published: 23 March 2017 David J. Wurm1,*, Johanna Hausjell1,*, Sophia Ulonska1, Christoph ...
Cloning and over-expression of penicillin G acylase in Escherichia coli BL21. Afr. J. Biotechnol. 9(18):2697-2701.Kafshnochi M, Farajnia S, Aboshof R, Babaei H, Aminolroaraee M (2010). Cloning and over-expression of penicillin G acylase in Escherichia coli BL21. AJB 9(18): 2697-...
elongatus PCC 7942 and E. coli BL21(DE3) was established for the production of isoprene with high cell concentration. The effects of S. elongatus PCC 7942 on E. coli BL21(DE3) were also investigated using methods of omics analysis. Materials and methods Strains, plasmids and media...
Through 2-DE based quantitative proteomics, the dynamic characteristics of overall proteome profiles of Escherichia coli BL21(DE3) were systematically analyzed in the presence of four different stressors. Dithiothreitol and 2-hydroxyethyl disulfide are a reducing and an oxidizing agent, respectively, which...
When producing recombinant proteins, the use of Escherichia coli strain BL21(DE3) in combination with the T7-based pET-expression system is often the method of choice. In a recent study we introduced a mechanistic model describing the correlation of the