(DE3) strains received: 25 October 2016 accepted: 20 February 2017 Published: 23 March 2017 David J. Wurm1,*, Johanna Hausjell1,*, Sophia Ulonska1, Christoph Herwig1,2 & Oliver Spadiut1,2 When
beta-galactosidaseOptimizationThe β-galactosidase-catalyzed conversion of lactose to galactooligosaccharides offers an attractive approach for the efficient production of galactooligosaccharides. In this study, the β-galactosidase fromBacillus circulanswas overexpressed inEscherichia coliBL21(DE3), and its ...
E. coli BL21 Star™ (DE3) is derived from the E. coli B strain, which was originally encapsulated; however, insertion of the IS1 element inactivated its native capsular biosynthesis. The gene cluster involved in transporting mechanism in regions I and III is still active, (Andreishcheva and...
(DE3) strains received: 25 October 2016 accepted: 20 February 2017 Published: 23 March 2017 David J. Wurm1,*, Johanna Hausjell1,*, Sophia Ulonska1, Christoph Herwig1,2 & Oliver Spadiut1,2 When producing recombinant proteins, the use of Escherichia coli strain BL21(DE3) in...
产品名称Escherichia coli BL21 Star(DE3)plysS 商品货号B80883 基因型F-ompT hsdSB (rB-mB-) gal dcm rne131 (DE3)pLysS (CamR) 基本信息BL21 Star (DE3)pLysS菌株来源于BL21 (DE3),含有rne131突变(RNaseE基因突变),RNaseE基因的突变降低了内源RNase的积累,增强了菌株细胞内mRNA的稳定性,从而提高了异源蛋白...
coli BL21 Star (DE3), separately. The recombinant strains were cultured to an OD600 of 0.6 and induced with 0.5 mM IPTG for 12 h at 25 °C. Cells were harvested and re-suspended in lysis buffer (50 mM Tris-HCl, 300 mM sodium chloride, 10 mM imidazole, pH 8.0). The...
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产品名称 Escherichia coli BL21(DE3) 商品货号 B98190 基因型 F- ompT hsdSB (rB- mB- ) gal dcm (DE3) 基本信息 本菌株适用于T7启动子(T7 promoter)调控目的基因表达的pET系列等原核表达质粒。 BL21缺失细胞质中的Lon蛋白酶(Lon protease)和胞外膜上的OmpT蛋白酶(OmpT protease),能有效避免重组表达蛋白...
图1 用于重组蛋白生产的E. coli菌株 (BL21 Star、Rosetta、SHuffle和Origami) 一、大肠杆菌BL21系列 来源于E. coliB株系,BL21菌株缺乏Lon蛋白酶(Lon protease)和外膜蛋白酶OmpT,Lon蛋白酶主要功能是降解外源蛋白,外膜蛋白酶OmpT主要功能是降解胞外基质蛋白,这两种关键蛋白酶的缺乏可有效避免重组蛋白的降解。BL21菌株...
BL21大肠杆菌,Escherichia coli BL21,BL21 英文名称: 总访问: 157 国产/进口: 国产 半年访问: 86 产地/品牌: 语纯生物 产品类别: 细胞株/菌种 规格: 冻干粉 最后更新: 2024-12-5 货号: 参考报价: 立即询价 电话咨询 [发表评论] [本类其他产品] [本类其他供应商] [收藏] 分享:微信新浪微博销售商...