Total protein was lysed by homogenization in RIPA buffer (Fujifilm) containing protease inhibitor cocktail (Nacalai Tesque) and Cryonase Cold-active Nuclease (Takara Bio), while total RNA was extracted using an
By visual examination of RNA-seq plots, we noted different lengths ofOAS1exon 3 (labeled as short and long; Fig.4a), created due to alternative splicing through cryptic acceptor (~15–25% of reads) and donor (~5% of reads) splice sites. Splice quantitative trait locus analyses showed incr...
The purified linear plasmids were transcribed in vitro using a RiboMAX™ Large Scale RNA Production System-T7 kit (Promega, Hilden, Germany) and thereafter purified using the RNeasy Mini Kit (Promega, Hilden, Germany). To calculate the copy numbers, the purified RNA was then spectrophometric...
Construction of Plasmids Column or gel purification of digested vector backbone was performed using the Qiagen QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) or the Gel Extraction Kit (Qiagen), respectively. Column purification of all PCR-amplified inserts and insert digests was done using...