Herein, we used single-cell transcriptome analysis to reconstruct the transcriptional network of AR in PCa. Our work shows AR directly regulates a set of signature genes in the ER-to-Golgi protein vesicle-mediated transport pathway. The expression of these genes is required for maximum androgen-...
suggesting that some vesicle-associated docking and fusion proteins are "programmed" during vesicle budding from the ER and direct downstream events that occur during fusion of these transport vesicles with the membranes of the Golgi.关键词: Animals Organelles Endoplasmic Reticulum Golgi Apparatus Cell ...
Notable changes included increased ribosomes and other RNA-binding proteins, and proteins involved in endoplasmic reticulum–associated degradation (ERAD), translation, ER-to-Golgi vesicle–mediated transport, and the response to ER stress. Proteins that decreased included signal recognition particle ...
ATF6α is a type II transmembrane protein that possesses a cytosolic N-terminus containing a bZIP motif [50]. ATF6α is located on the ER membrane with BiP bound to its Golgi localisation sequences. Under ER stress BiP is released from ATF6α, allowing translocation to the Golgi apparatus ...
Subsequent transport to the Golgi is mediated by tubulovesicular membranes. The anterograde membrane flow is compensated for by a retrograde pathway, which, in addition to the recycling of membrane and proteins to the endoplasmic reticulum, may play a role in anterograde cargo concentration....
(GO)59, and observed these genes were highly associated with ER stress amelioration, including molecular chaperones and ERAD. The 41 MIN6-specific genes were enriched for functions in ER-to-Golgi vesicle transport (Supplementary Fig.4d). This analysis revealed a unique adaptive event in MIN6 ...
We review the mechanisms that govern coat recruitment to the membrane, cargo capture into a transport vesicle, and accurate delivery to the target organelle. 展开 关键词: Transport Vesicles Endoplasmic Reticulum Golgi Apparatus Animals Protein Transport ...
A cell-free vesicle fusion assay that reproduces a subreaction in transport of pro-伪-factor from the ER to the Golgi complex has been used to fractionate ... Barlowe,C. - 《Journal of Cell Biology》 被引量: 360发表: 1997年 SNARE-Mediated Retrograde Traffic from the Golgi Complex to th...
mSigR1 impairs ER to Golgi transport. (a) Cos7 cells showing abnormal SigR1 accumulations (arrows) after mSigR1 transfection and normal ER localization of SigR1 (right) after wtSigR1 transfection. Scale bar, 15 μm. (b) Cos7 cells were co-transfected with VSVG-GFP together with pcDNA...
Such aggregation causes retention of secretory cargos at ERES and therefore delays ER-to-Golgi transport, providing a link between TDP43 aggregation and compromised cellular function in ALS patients.Similar content being viewed by others M6A reduction relieves FUS-associated ALS granules Article Open ...