Protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus may be monitoredKeller, D SBaker, DHicke, LRexaeh, MSehleyer, MSchekman, RKabeenell, A K
Sorting membrane proteins by size in the Golgi De Caestecker and Macara find that the sorting of membrane proteins in the Golgi relies on a size filter that enables correct localization of proteins with a short cytosolic domain to the apical membrane. ...
The main step in protein secretion is protein transport from endoplasmic reticulum (ER) to Golgi and further transport to the extracellular space. Increasing protein secretion can significantly enhance protein production. Moreover, the secretory system of recombinant proteins inS. cerevisiaeis beneficial f...
Bos1p, a membrane protein required for ER to Golgi transport in yeast, co-purifies with the carrier vesicles and with Bet1p and the ER membrane. BOS1 and BET1 are required for transport from the ER to the Golgi complex in yeast and genetically interact with each other and a subset of ...
Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast. Sec12p and Sar1p are required for the formation of transport vesicles generated from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae. ... ...
1f). ERES are specialized ER domains that form transport carriers with protein cargoes destined for secretion via the Golgi apparatus53. Our screen identified most ERES proteins as required for GPAT4 targeting to LDs, including Sec12, Sec16, Tango1 and COPII coat components (Sar1, Sec23, Sec...
Introduction The Protein Processing Assembly Line Polypeptide Production The Endoplasmic Reticulum: Polypeptide Folding and Modification Overview of the ER Lumen Folding in the ER Glycosylation in the ER Glycosylphosphatidylinositol-Linked Proteins Membrane Traffic: ER to Golgi and Beyond The Golgi Apparatus ...
(SCAP; pink). When cells are exposed to sterols (such as cholesterol), sterol synthesis is prevented because sterols bind SCAP, which changes its conformation. As a result, SCAP–SREBP complex formation is prevented, SREBP translocation from the ER to the Golgi apparatus is inhibited, and ...
When proteins are produced in the ER, they have to pass through the Golgi apparatus for processing before being released into the cell to be used. Here, the protein released from the ER pass through the cis face to enter the Golgi apparatus. It's important for these molecules to pass ...
Whereas the former is targeted for lysosomal degradation, CI-MPR is transported to the Golgi apparatus and cell surface for recycling.83 This natural process is harnessed to generate the first LYTAC molecules, which consist of a small molecule or antibody fused with synthesized a CI-MPR-targeting...