For each sample, equal amount of RNA was pooled from different tissue regions into a tube. 500 ng of RNA (in a volume of 50 µL) was used to prepare the library using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, USA) accor...
Furthermore, our analysis of labelled cell clusters reveals oriented patterns of growth in the DPW directed towards the arterial pole of the heart. Similarly, global tension patterns orient cell stretching patterns and cell division to determine tissue shape in 10 NATURE COMMUNICATIONS | 8:14770 | ...
Depending on the signals received by a cell from its surrounding environment within a tissue and the cell's internal gene circuitry, the EMT program generates cells that transition through various intermediate phenotypic states as they progress from an epithelial to a mesenchymal state. During this ...
Since these bacteria were first isolated from the lung tissue of sheep with lung adenoma in 1963 [1], the mechanisms underpinning its pathogenesis have been extensively investigated. Studies have been demonstrated that airway epithelial cells are the main targets of M. ovipneumoniae infections and ...
Functional investigations into the splicing isoforms of PKHD1 is considerably hampered by the lack of well- validated immunoreagents as well as by the tissue-restricted expression pattern of PKHD1. The lack of PKHD1 expres- sion in tissues that are usually available for analysis such as ...
A. Diagram showing proteins associated with the various classes of cell-cell junctions. Circled proteins were labelled in the Bio ID experiment. B. mRNA expression for junctional proteins in the TCGA. Note: Claudin-4, claudin-3, tight junction protein 1 (TJP1), E-cadherin (CDH1), and ...
Secondary antibodies used were HRP-labelled anti-rabbit IgG (1:3000, #7074, Cell Signalling Technology, Danvers, MA, USA) and HRP-labeled anti-mouse IgG (1:3000, #7076, Cell Signalling Technology).Quantitative analyses of EVs We quantified the production of EVs mainly by EV-specific protein ...
Estrogen-dependent proliferation is one of the defining features for ER+ breast cancer, however this has been considered a newly acquired trait because ER+ cells in normal non-pregnant breast tissue rarely proliferate. It will be interesting to explore to what extend ER+ cancer cells hijack this...
Single cells (1–2 × 105) of USCs were treated with DNase I to remove trace DNA contamination from the Matrigel. Genomic DNA (gDNA) was prepared from the cells using DNeasy Blood & Tissue kit (Qiagen, 69504). Using 200 ng of gDNA and 0.4 ng lambda, DNA was bisulfite trea...
Stem/progenitor cells of the airway have received enormous attention because they may be good candidates for cell therapy or tissue engineering. The ability to generate airway epithelial cells (AECs) from embryonic stem cells20,21 and induce pluripotent stem cells22,23 in vitro has provided hope ...