The somitoids created with different CHIR concentrations were labeled with MULTI-seq barcode oligonucleotides for sample multiplexing as described (Supplementary Figs. 20–22)46. Briefly, 5 × 105 cells from the prepared single-cell suspension were resuspended in Cell Prep Buffer (PBS(-) contai...
Cell polarity and cancer-cell and tissue polarity as a non-canonical tumor suppressor. J Cell Sci. 2008;121:1141–50. Article CAS PubMed Google Scholar Meulmeester E, ten Dijke P. Integration of transcriptional signals at the tumor cell invasive front. Cell Cycle Georget Tex. 2010;9:2499...
To assess the changes in lung tissue cell composition after SiO2exposure, we performed scRNA-Seq on lung tissue samples. A total of 20 cell type were identified and viewed in two dimensions using t-distribution random neighborhood embedding (t-SNE) (Fig.S1A). To investigate the role of alveo...
Given the high expression of wound response genes in the tissue adjacent to cancer [58] and the important role of HGF in normal ductal morphogenesis and invasive breast cancer, a better understanding of HGF/MET in progression of basal-like breast cancers is important. Future work should focus ...
Given DVF-mediated exacerbation of colitis was not due to alteration of gut microbiota, RNA‐seq of colon tissue from DVF or PBS-treated mice with colitis were performed to investigate the effect of DVF on colitis. Heatmap for differential gene expression between two groups was displayed in Fig...
Tissue sections were incubated with primary antibody overnight at 4 °C; secondary antibodies and Streptavidin-HRP for 1 h at room temperature. The slides were scanned through the Nanozoomer S60 digital slide scanner slide scanner (Hama- matsu; RRID:SCR_022537). Statistical analyses Survival...
4A). Pathological evaluation of mouse lung tissue showed that the score of pathology (Fig. 4B) was increased in TWMP-treated mice. After that, pulmonary parenchymal area (PA), average vessel wall thickness, number of alveolar septal (NS) were analyzed through histomorphological measurement. PA ...
pylori positive tissue compared to non-infected tissue (Figure 6B). To corroborate our results in another patient cohort, we checked the expression of NRF2 using the UALCAN database, which confirmed that NRF2 expression is decreased in H. pylori-infected gastric mucosa (Figure 7A). However, ...
E-cadherin downregulation is thought to be one of the main reasons for loss of cell-cell-contacts and therefore tissue integrity in cancer [50]. However, others have reported that modulation of cell-cell adhesion by estrogen signaling occurs through E-cadherin membrane redistribution and adherens ...
It is obvious that TRPA1-IR was located in most of E-cadherin-positive epithelial cells on the colonic tissue sections (Figure 3I, upper panels). Notably, it seems that C. rodentium infection led to the reduced expression of TRPA1 in E-cadherin-labeled epithelial cells (Figure 3I, lower ...