All these parameters were calculated by an automated software. Immunofluorescence staining with human synovial slides Epitopes were retrieved from deparaffinized sections using a heat-induced method. As described before19, sections were alternatively bathed in boiling citrate buffer (10 mM citric acid ...
Eosinophil and Treg numbers were positively correlated in WT mice, and Tregs were reduced—also as quantified by automated microscopy—upon eosinophil depletion (Fig. 1h, i). The phenotype of eosinophil-depleted Eo-CreDtr mice was recapitulated in PHIL mice, which transgenically express diphtheria ...
The peripheral blood analysis for the complete blood count test was performed on an automated hematology analyzer XE-5000™ (Sysmex, Kobe, Japan). 2.9. Granulocyte Isolation from Human Peripheral Blood and Eosinophil Enrichment Approximately 24 mL of peripheral blood was collected into sterile Ethylen...
2.8. Analysis of Peripheral Blood Cells The peripheral blood analysis for the complete blood count test was performed on an automated hematology analyzer XE-5000™ (Sysmex, Kobe, Japan). 2.9. Granulocyte Isolation from Human Peripheral Blood and Eosinophil Enrichment Approximately 24 mL of ...
A UniCel® DxH 800 Coulter® Cellular Analysis System automated hematology analyzer (Beckman Coulter, Miami, FL, USA) was used for the complete blood count test. Whole eosinophils’ isolation from peripheral blood procedure is described in our previous publication [16]. 2.7. Combined Cell ...
IL33 activation, which increased the eosinophil count and secretion of MBP and IFNγ. In particular, a high IL33 level was associated with prolonged OS among liver cancer patients. These results suggest that KRG enhances the immunotherapeutic effects of NK cells in the metastatic liver cancer ...