Eomes对CD4+ T细胞的调节作用尚在研究中,但Eshima等已发现仅单独引入Eomes基因就可通过穿孔素和FasL途径使已分化完全的 Th细胞功能向CTL细胞功能转变 [7]。1.2T-bet和Eomes对CD8+ T细胞的调控作用 CD8+ T细胞在机体抗感染和抗肿瘤免疫应答中有不可替代的作用。 T-bet和Eomes共同调控 CTL靶基因的转录 [8]。T-...
【摘要】本文使用地塞米松处理健康人外周血单个核细胞(PBMCs)72 h后,探究其对CD4+T细胞及其亚群的频数、增殖、凋亡比例;功能性细胞因子及关键转录因子表达的影响.结果显示,地塞米松组CD4+T、2型辅助性T细胞(Th2)、Th17细胞比例增加(P<0.01,P<0.05,P<0.01),Th1细胞比例降低(P<0.01).Th1细胞上γ-干扰素(IFN-γ...
Fig. 5 EOMES expression is sufficient to drive TIMtranscriptional program.aRepresentative flow cytometry plots of total thymocytes from WT andEomesTgmice showing DN, DP, CD4SP, and CD8SP proportions. Histograms indicate the frequency of each population and their EOMES expression (MFI).bExpression of...
Anti-Dog CD4 -PE-Cyanine7/小鼠抗狗CD4,PE-Cyanine7 产品类型:流式抗体 规格:25 Tests,100 Tests AD0404125 ,AD0404110 我要询价 添加 RevertAid First Strand cDNA Synthesis Kit/反转录第一链cDNA合成试剂盒 cDNA合成试剂盒; 42°C最佳活性温度; 完全组份试剂盒; 高效合成长达13kb cDNA ...
Overexpression of EOMES rescues IFN-γ production by T-BET deficient CD4 + T cells33,34. Moreover, the combined deletion ofT-BETandEOMESleads to a complete deficiency in NK cells while single mutations of either gene leads to a partial defect22. Together with the similarity in EOMES and...
本文使用地塞米松处理健康人外周血单个核细胞(PBMCs)72h后,探究其对CD4^+T细胞及其亚群的频数,增殖,凋亡比例;功能性细胞因子及关键转录因子表达的影响.结果显示,地塞米松组CD4^+T,2型辅助性T细胞(Th2),Th17细胞比例增加(P<0.01,P<0.05,P...
摘要: 本文使用地塞米松处理健康人外周血单个核细胞(PBMCs)72 h后,探究其对CD4+T细胞及其亚群的频数、增殖、凋亡比例;功能性细胞因子及关键转录因子表达的影响.结果显示,地塞米松组CD4+T、2型辅助性T细胞(Th2)、Th17细胞比例增加(P<0.01,P<0.05,P<0.01),Th1细胞比例降低(P<0.01... 查看全部>> ...
AF700 rat anti-mouse CD4; clone RM4-5; 1:100 BD Biosciences Cat# 561025;RRID: AB_396956 APC-Cy7 rat anti-mouse CD4; clone RM4-5; 1:100 BD Biosciences Cat# 565650;RRID: AB_2739324 PE-Cy7 rat anti-mouse CD4; clone RM4-5; 1:100 BD Biosciences Cat# 561099;RRID: AB_394461 PerCP...
目的:探讨转录因子T-bet和Eomes对不同亚类人T细胞产生IFN-γ的调控作用.方法:化学合成针对人T-bet和Eomes基因的小干扰RNA(siRNA),转染体外培养的CD3单克隆抗体(mAb)活化的αβT细胞和Mtb-Ag活化的γδT细胞,利用流式细胞仪分选纯化CD4~+,CD8~+T细胞和γδT细胞,半定量RT-PCR检测CD4~+,CD8~+T淋巴细胞中T-bet...
Intestinal NK cells express tissue-residency markers.aviSNE plots of combined flow cytometric data visualizing CD103, CD49a, and CD69 expression on infant and adult NK cells from epithelium (EP) and lamina propria (LP) intestinal tissues. Cell density of clusters is shown in first row. Expressi...