View Solution Enzyme used in recombinant DNA technology (RDT) is View Solution Which enzyme is responsible for linking the fragments of DNA Or The DNA joining enzyme, required on recombinant DNA technology View Solution Recombinant DNA technology developed during ...
The range of time-scales involved in substrate turnover step of enzyme catalyzed reactions and internal protein dynamics are similar. Note the universal frequency factor (kBT/h), which is commonly used in transition state theory;kBis the Boltzmann's constant,Trepresents the ambient temperature andhi...
In the other three cases, where gentler purification methods were used or no purification was necessary, the amount of 3Fe species present correlated with the amount of cross-linked species. The remaining iron in the spectrum was split between ferrous iron that corresponded to the iron lost from...
The phylogenetic relation- ship we demonstrate between the LRAT family of enzymes and thiol peptidases shows that this catalytic triad was derived only once in evolution and re-used for different mechanistically similar reactions such as acyl transfer, transacylation or peptide-bond hydrolysis. The ...
in cells. It is an objective of the present invention to provide novel split-firefly luciferase (split-Fluc) sensors that can be used for measuring PPIs and methods of designing said sensors, as specified in the independent claims. Embodiments of the invention are given in the dependent claims...
Interaction with the Zip domain was used as a negative control. The β-galactosidase activity values are the average of at least three independent experiments and are expressed in Miller units. A statistical test was performed by the no parametric Wilcoxon/Mann–Whitney method. Two stars stand...
Response of Soil Microorganisms to Herbicides The study has shown that the tested herbicides stimulated the growth of organotrophic bacteria, regardless of the dose used. Tezosar 500 SC applied to the soil in the RD dose caused an increase in the number of fungi, whereas Sulcogan 300 SC and...
In enzyme-based biosensors, the techniques of enzyme immobilization are highly significant, due to relative instability of the mobile enzyme, the difficulty in the active recovery of the enzyme, etc. [1]. On the other hand, the immobilized enzymes can be used repetitively and continuously, and ...
Introduction Cu nanoparticles (Nano-copper) represent a promising modality in biomedical research and clinical applications. Nano-copper has shown great potential as an antibacterial agent and additive in animal feed [1–3]. Furthermore, nano-copper has been widely used in industry, e.g., as an...
Although in the literature the term endolysin is very frequently shortened to lysin, this simplification will not be used in this review to avoid confusion. Likewise, VALs are also known as virion-associated peptidoglycan hydrolases (VAPGH), tail-associated muralytic enzymes (TAME), tail-...