酶的底物特异性(substrate specificity) 酶有一个特殊的区域称为活性部位(active site)。由于substrate的形状与active site的形状高度契合(fit),因此substrate能够与active site特异性地结合,形成酶-底物复合物(enzyme-substrate complex)。Enzyme-substrate complex形成会涉及到一些化学基团(chemical groups)之间的相互作用。
pH等影响)6. Enzymes possess specificity (酶具有特异性)这是因为enzyme有一个特殊的区域称为活性部位(active site).由于substrate的形状与active site的形状高度
酶的特异性(Enzyme specificity)及活性位点的诱导契合模型(induced-fit model) 与工业化中用到的无机催化剂不同,酶在发挥作用时有高度的专一性。酶的活性位点中的氨基酸排布方式刚好使得能其与底物的某些化学基团发生结合,形成enzyme–substrate complex。 在enzyme–substrate complex形成之后,酶的活性位点结构会发生稍微...
The new method shows promise of developing into a useful probe for the quantitative study of biochemical systems.Computation of enzymesubstrate specificity
While the substrate specificity is strict for the terminal sugar in the substrate, the enzymes display a broad specificity toward the aglycone and the leaving group in the substrate. In fact, the substrates for the glycosidases that are frequently used in an activity assay are synthetic glycosides...
An enzyme will interact with only one type of substance or group of substances, called thesubstrate, to catalyze a certain kind of reaction. Because of this specificity, enzymes often have been named by adding the suffix “-ase” to the substrate’s name (as inurease, which catalyzes the ...
Köhn, M. et al. A microarray strategy for mapping the substrate specificity of protein tyrosine phosphatase.Angew. Chem. Int. Ed.46, 7700–7703 (2007). ArticleCASGoogle Scholar Feilner, T. et al. High throughput identification of potential Arabidopsis mitogen-activated protein kinases substrate...
M. I. Page, The specificity of enzyme-substrate interactions. In Accuracy in Molecular Processes (Eds T. B. L. Kirkwood, R. F. Rosenberger and D. J. Galas), pp. 37-66. Chapman & Hall, London (1986).RYAN, M. D.; BELSHAM, G. J.; KING, A. M.: ' Specificity of enzyme-...
R. 1980. Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi. J. Biol. Chem. 255:839-840.Drapeau GR: Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi. J Biol Chem 255:839, 1980...
Residues in the binding pocket, T199, L202, M259, and L261, of 2-pyrone synthase (2PS) that determine substrate specificity and/or control product size are selected based on sequence homology as described8. To recombine homologous mutations, these residues are combinatorically mutated to either...