reaction with later reagents to form a colored compound, is added. Again, the well is rinsed to remove any unbound secondary antibodies and the plate is incubated with a colorless substrate that will become a colored reaction product in the presence of labeled antibody. A microplate reader, ...
T4 DNA ligase recognizes these nicks or breaks in the backbone and uses energy from the hydrolysis of ATP to drive the ligation reaction. 1.6 Making a Restriction Map A diagram that shows the location of restriction enzyme cut sites on a segment of DNA is known as a restriction map. Today...
In the presence of ZEN, it will preferentially combine with the aptamer, destroying the hydrogel structure and then releasing the AuPt nanozymes to complete the catalytic reaction. Therefore, a highly sensitive colorimetric method for the determination of ZEN was established with a LOD of 0.6979 ng...
An enzymic reaction is utilized to yield color and is used to quantify the reaction, through use of an enzyme labeled reactant. A very brief definition of terms used in ELISA is needed. 12 TERM Definition TERM Definition This is usually a microtiter plate well. Specially prepared ELISA Solid...
3c, maltodecaose (G10) is the minimum glucan length necessary for the RpBE reaction to be observed. We, therefore, employed both G7 and G10 as substrates to dock with RpBE, and compared their binding poses. Fig. 3: Schematic diagram of the whole process for generating the branching ...
we have been able to significantly push the optimal catalytic capability of these promising IL-based microreactors. As exemplified by a lipase driven kinetic resolution reaction, reinforced activity and thermostability are validated in an industrially preferred continuous-flow catalysis manner. More interesti...
AuNCS were synthesized by using a galvanic replacement reaction between Ag nanocubes (AgNCs) and HAuCl4as previously reported [56,57]. To prepare AgNCs, EG (5 mL) was heated to 150 °C under stirring. NaHS solution (3 mM), HCl solution (3 mM), PVP solution (20 mg/mL) and CF3COO...
Requirement of lectin domain in efficient enzyme reaction To investigate the functions of the GnT-IVa lectin domain in more detail, we purified soluble wild-type enzyme and its D445A mutant (Fig.5a, b) and measured their enzymatic activity. The activity of D445A was reduced to ~62% of th...
CMFDA-labeled Jurkat (A) or THP-1 (B) cells were then added. After incubation for 60 min at 37°C, nonadherent immune cells were removed by washing with HBSS and the monolayer-associated Jurkat cells or THP-1 was counted. Values are showed as the percent inhibition. All treatments were...
In the biotransformation of lipophilic xenobiotics, microsomal oxidation is the most prominent reaction where microsomal enzymes associated with smooth ER of hepatocytes are involved and the enzymecytochrome P450, aheme protein, which is a part of an enzyme system termed as mixed functionoxidase(MFO) ...