Reaction between 1- and 2-naphthol with diazotized sulfanilic acid leads to four different dye products, each formed at rates that differ from one another by several orders of magnitude. A traditionalbatch reactorleads to a mixture of dyes, whereas when the reaction is performed in a HEX reac...
9b, c). We showed that catalytically incorporated azidobutyrylated histones can be labeled by alkyne-conjugated TAMRA via click chemistry, visualizing endogenous histones in THP-1 cells (Supplementary Fig. 9d). To address the cell-type selectivity of the catalytic histone acetylation reaction, we ...
Chemical labeling usually targets either intact proteins or biochemically digested peptides, and is achieved via chemical bonding with amino acid side chains (e.g., lysine and cysteine), primary amines in the n termini of proteins and peptides or by catalysis with an isotope reaction material, s...
increasing the temperature increases the rate of the reaction to a certain extent, but precaution is needed while increasing the temperature of the reaction to avoid accidents. For example, the dissolution of sugar in water takes place faster when the water is hot as compared...
3a). Euchromatin would be labeled if dUTP-Cy5 was incorporated into DNA during the early S phase, appearing as hundreds of smaller foci scattered throughout the nucleoplasm (Fig. 3b). We observed clear co-localization between dUTP-Cy5-labeled heterochromatin and adriamycin (Fig. 3a, arrows), ...
Autonomous reaction network exploration algorithms offer a systematic approach to explore mechanisms of complex chemical processes. However, the resulting reaction networks are so vast that an exploration of all potentially accessible intermediates is co
To test the optional pH value for PnGH1 activity, assays were performed in different reaction buffers in pH values ranging from 4.0 to 6.0 (citric acid–sodium citrate buffer), 6.0–8.0 (K2HPO4–KH2PO4 buffer), 7.0–9.0 (Tris–HCl buffer) and 9.0–11.0 (Na2CO3–NaHCO3 buffer), ...
Fascinatingly, benzimidazole-47-dicarboxylic acid (H2bidc) was produced within the reaction environment, with H2BTDC as the initial compound. Reactant concentrations and solvent choices enable the controlled self-assembly of MOFs with diverse topological frameworks. JXUST-20 and JXUST-21 are found to...
The high temperature could lead to the chemical reaction in the adjacent aera if it is larger than the minimum chemical reaction temperature. Sometimes the chemical reaction could be self-sustained and the spherical flame is formed. If the chemical reaction ends before the combustible premixed ...
Autonomous reaction network exploration algorithms offer a systematic approach to explore mechanisms of complex chemical processes. However, the resulting reaction networks are so vast that an exploration of all potentially accessible intermediates is co