Villaume I, Thomas D, Legoy M (1990) Catalysis may increase the stability of an enzyme: the example of horse liver alcohol dehydrogenase. Enzyme Microb Technol 12:506-509Villaume, I., Thomas, D., and Legoy, M. D. (1990), Enz. Microb. Technol. 12 , 506–509....
Entropy and Enzyme Catalysis 来自 掌桥科研 喜欢 0 阅读量: 54 作者:?qvist, Johan,M Kazemi,GV Isaksen,BO Brandsdal 摘要: ConspectusThe role played by entropy for the enormous rate enhancement achieved by enzymes has been debated for many decades. There are, for example, several confirmed ...
General chemical strategies for PSRs with unactivated ketones can involve Brønsted acid catalysis3,4, Lewis acid catalysis5,6or use of fluorinated solvents7. However, to our knowledge, there are no general methods for stereoselective chemical PSRs with ketones. Importantly, efficient routes to chira...
Our approach thus provides a general way to map the catalysis of a mutated enzyme, to characterize the range of products it generates, and to recover the sequences of variants with desired activities. Fig. 1: Metabolic biosensing with microscale mass spectrometry provides a general strategy for ...
enzyme inhibitors repress an organism’s vital physiological functions. Many toxic substances, including pesticides, such nerve gases as lewisite, and such respiratory toxins as cyanides and hydrogen sulfide, have a toxic effect owing to the inhibition of enzymes, for example, the enzyme cholinesteras...
Dienamine Catalysis: An Emerging Technology in Organic Synthesis High-yielding asymmetric synthesis of bioactive molecules with the aid of mild catalysis is an efficient approach in reaction engineering. In the early day... Dhevalapally,B.,Ramachary,... - 《Cheminform》 被引量: 383发表: 2011年...
(enantio) selective reaction is often desired for use in white biotechnology.1,2Detecting catalysis is important because the mere presence of the enzyme, for example, as a protein with the expected mass as a band in agel electrophoresisor as a peak inmass spectrometry(MS), is not sufficient...
Enzyme catalysis initially involves the substrate binding to the active site of the enzyme to form an enzyme–substrate complex as an intermediate state. As the reaction progresses, the enzyme detaches from the products without being consumed itself [3]. The active sites of an enzyme consist of...
1 •Kineticpropertiesofenzymes:–Increaserateofbiologicalreactionswithoutalteringreactionequilibria–Acceleratereactionsthroughstabilizationoftransitionstates–Theenzyme-substratecomplex:firststepincatalysis–Theenzymeactivesite–Michaelis-Mentenmodelofenzymekinetics(VmaxandKm)–Enzymeinhibition(typesofinhibitors,applicationsof...
Metal Ion Catalysis几乎1/3的酶催化活性需要金属离子。金属离子主要以三种方式三种方式参与催化过程:1. 与底物结合,定向底物,定向底物,便于反应;2. 通过金属离子氧化态的变化介导氧化还原反应氧化还原反应,如Fe2+与Fe3+等;3. 静电稳定或者掩盖电荷稳定或者掩盖电荷。(六)酶作用机制举例1. 溶菌酶:活性部位在酶分子...