Base SequenceBlotting, NorthernCell NucleusChloramphenicol O AcetyltransferaseA strong T cell-specific enhancer is located 3' to the human CD2 gene. Six sequences within this enhancer are bound by proteins present in T cell nuclear extracts. These sequences share homology with sequences bound by ...
The detailed primer sequences are exhibited in Supplementary Data 1. Flow cytometry analysis Tumors were excised and mechanically minced, and treated with collagenase (0.2 mg/ml, Sigma) and DNase I (1U/ml) for 15 min at 37 °C. Cells were passed through 70 μm filter. And ...
Enhancers arecis-regulatory DNA sequences that are bound and regulated by transcription factors (TFs) and chromatin regulators in a highly cell-type and temporal-specific manner. Putative enhancers are operationally identified using epigenetic signatures including chromatin accessibility (DNase I hypersensitiv...
by being composed of multiple binding sites for transcriptional activator proteins. Many enhancers control gene expression in a cell type-specific fashion. Expression of one and the same gene can be controlled by several remote enhancers. Conversely, a given enhancer may also serve to activate more...
these enhancers are expected to display higher evolvability, and thus exhibit lower conservation. It is possible for such enhancers to display similar TF occupancy despite having diverged sequence motifs, and dissimilar TF occupancy despite conserved sequences [75]. On the contrary, the “enhanceosome...
Immunoglobulin Enhancer Binding Protein In subject area: Biochemistry, Genetics and Molecular Biology Nuclear factor-κ B (NF-κB) is a collective designation for a family of dimeric transcription factors which include p50/p105 (NFκB1), p52/p100 (NFκB2), c-Rel, p65 (RelA), and RelB. ...
The shRNA sequences are listed in Supplementary Data 4. RNA extraction, reverse transcription and quantitative PCR Total RNA was extracted using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversely transcribed into cDNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, ...
These enhancers are able to drive gene expression during differentiation coincident with loss of H3K27me3 and gain of H3K27ac. Intriguingly, poised regions remain bound by the histone acetyltransferase p300 in the pluripotent state, suggesting the existence of a mechanism to prevent premature ...
are bound by activator proteins and stimulate the rate of transcription, while silencers were bound by repressor proteins and decrease the rate. In certain cases when the interactions between enhancers/silencers and promoters are unwanted, insulators can block their interactions [4]. Bound by tissue-...
2a) that are bound by the ERα transcription factor and at the same time exhibit the active enhancer mark H3K27Ac upon treatment of the MCF7 cell line with estrogen, as identified via ChIP-seq experiments by Li et al.10. We sought to determine the sequence features that distinguish the ...