In recent years, increasingly more non-coding RNAs have been detected with the development of high-throughput sequencing technology, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), small nucleolar RNAs (snoRNAs), and piwi-interacting RNA (piRNAs). The disco...
In this study, we created a catalogue of eRNA regions using genome-wide chromatin immunoprecipitation-sequencing (ChIP-seq) and RNA sequencing (RNA-seq) data across 50 human cell and tissue types. We characterized these eRNA regions and extended our understanding of their functionality by analysing ...
eRNA-protein interactions Enhancer RNAscan be involved in regulation of gene expression via interactions with different proteins, such as theMediator complex, transcription factors,cohesins, or RPII. Similar tolncRNAs, eRNA may provide baits and scaffolds or even traps for their interactors. Some eRNAs...
eRNA可能参与增强子-启动子环维持或招募特定转录因子,那么可以通过预测eRNA相关增强子、靶基因启动子的转录因子结合, eRNA相关增强子与eRNA靶基因启动子如果具有相同的转录因子结合,并且实验数据证明增强子和启动子具有DNA交互,即认为此eRNA参与了其相关增强子与靶基因启动子环维持或参与招募此转录因子。共挖掘到34个enhancer...
Furthermore, eRNAs, like other RNA transcripts, can be visualized by in situ hybridization, using complementary RNA probes labeled with biotin or fluorescein [25]. When two or more RNA probes are used to simultaneously detect the eRNA and nearby protein-coding transcript(s), the dynamic relations...
eRNA据估计在人类细胞和组织内有超过六万个【8】。在增强子激活基因表达的作用过程,eRNA的功能已有报道,但是作用机制仍然未明【7】。 2021年8月9日,来自美国德克萨斯大学休斯敦健康科学中心麦格文医学院的李文博团队在Molecular Cell杂志...
We propose that further studies of eRNAs in cancer will offer a promising "eRNA targeted therapy" for human cancer intervention.doi:10.1080/15476286.2020.1712895Joo-Hyung LeeFeng XiongWenbo LiRna Biology
Noncoding RNAs (ncRNAs) direct a remarkable number of diverse functions in development and disease through their regulation of transcription, RNA processing and translation. Leading the charge in the RNA revolution is a class of ncRNAs that are synthesiz
eRNA可能参与增强子-启动子环维持或招募特定转录因子,那么可以通过预测eRNA相关增强子、靶基因启动子的转录因子结合, eRNA相关增强子与eRNA靶基因启动子如果具有相同的转录因子结合,并且实验数据证明增强子和启动子具有DNA交互,即认为此eRNA参与了其相关增强子与靶基因启动子环维持或参与招募此转录因子。共挖掘到34个enhancer...
introduction of double-stranded RNA interferes (RNAi) with native gene function, mimicking either a strong loss-of-function or null phenotype[10]. Furthermore, gene knock-outs can also be generated[11,12]. A key benefit for cell-biological studies is the transparent cuticle that allows visualiza...