The extent of cell death was quantitated with crystal violet viability assays and apoptosis was confirmed by morphological and fluorescence studies. Serum-free media conditioned by apoptotic EC were used to serum-starve vascular smooth muscle cells (VSMC). Conditioned medium was found to significantly...
It was the aim of this study to investigate endothelial cell apoptosis and angiogenesis focussing on the role of COX-2, prostaglandins and endogenous apoptotic inhibitors in these pathways. Endothelial cell apoptosis was assessed by chromatin condensation, DNA fragmentation and caspase activation. ...
Necrotic cell death only became evident at menadione concentrations ranging from 75–100 μM for both cell types. The timing of the apoptotic response to a 1 h menadione exposure was very specific. For both EC types, peaks of apoptosis occurred in two distinct waves, at 6–8 and 18–22 ...
Identification of a novel senolytic agent, navitoclax, targeting the Bcl-2 family of anti-apoptotic factors. Aging Cell 15, 428–435 (2016). Article CAS Google Scholar Caland, L. et al. Knockdown of angiopoietin-like 2 induces clearance of vascular endothelial senescent cells by apoptosis, ...
In vitro studies using endothelial cells from aortic or pulmonary origins and from cell lines show that Aβ is toxic.89,90Furthermore, these Aβ can cause cerebral endothelial cells to become apoptotic.91 Vascular endothelial cells also express α7receptor proteinand mRNA. As previously described,...
Endothelial cell activators in COVID-19 The pathophysiological activation of EC in the context of COVID-19 is triggered by a variety of stimuli, including pro-inflammatory cytokines, vasoactive compounds, components of the immune system, or even by direct actions of SARS-CoV-2 and its isolated ...
It has been postulated that apoptotic endothelial cell-derived micro particles (EMPs) might be a trigger of endothelial cell dysfunction and as well as a mediator on vascular repair. Moreover, the imbalance in circulating number of various types of EMPs may influence the risk of transformation of...
Thus, in endothelial cells p38α mediates apoptotic signaling, whereas p38β and p38γ transduce survival signaling. TGF-β1 activation of p38α is mediated by VEGF, which in the absence of TGF-β1 activates p38β. Therefore, these results show that TGF-β1 induces endothelial cell apoptosis ...
The clonal expansion presupposes an increased cell proliferation ofCcm3−/−cells within the lesions, as we have previously shown in vivo24.Ccm3is known to have a pivotal role in regulating cell survival and cell death, and anti-apoptotic25,26,27as well as pro-apoptotic28,29,30,31functi...
This S0.5 was then centrifuged at 14,000× g for 15 minutes (Eppendorf F-45-24-11 rotor, rotor k-factor 830, 4 °C) to collect the pellet P14, which was assumed to contain mainly larger EVs, enriched in shedding microvesicles but also apoptotic bodies and cell debris. The ...