ANTIBODY formationGERMINAL centersB cellsRESPIRATORY infectionsEndophilin A2, the sole endophilin A family member expressed in hematopoietic cells, regulates various aspects of membrane dynamics, including autophagy and endocytosis. Recent studies in rodents highlight the essential role of endophilin A2 in ...
We have identified a novel tumor marker, antibodies to endophilin A2 (EA2), to improve early diagnosis of breast cancer. Methods Antibody levels of EA2 were analyzed in sera of patients with cancers of different origins and stages by indirect enzyme-linked immunosorbent assay (ELISA). Diagnostic ...
d Pull-down using GST-SH3 domains of Endophilin A1, A2, or A3 and cell extracts expressing the indicated EGFP-tagged CRMP proteins. GST was used as negative control. Binding proteins were detected by immunoblotting with an anti-EGFP antibody. input lanes correspond to 5% of the cell ...
Ultrathin sections (70 nm) were collected on 200-mesh nickel grids coated with Formvar membrane, rinsed and blocked with BSA/PB (1% BSA in 0.1 M PB) and incubated with anti-en- dophilin A1 antibody (1:5) and/or anti-p140Cap antibody (1:10) in BSA/PB overnight at 4 °C, ...
Anti-GFP immunoprecipitation from HeLa cell lysate probed with anti-RFP antibody is shown (three experiments). (D) FBXO32-GFP-coupled beads pull-down purified endophilin-1. (E) MEF overexpressing FBXO32-EGFP (left) and endophilin A2-RFP (middle); magnified in the inset. Note that FBXO32...
kl901Hu01 激活素受体样激酶1(ALK1)多克隆抗体 Polyclonal Antibody to Activin Receptor Like Kinase 1 (ALK1) kl902Hu01 心肌钙黏蛋白(CDHH)多克隆抗体 Polyclonal Antibody to Cadherin, Heart (CDHH) kl903Hu01 纤维胶凝蛋白3(FCN3)多克隆抗体 Polyclonal Antibody to Ficolin 3 (FCN3) ...
The requirement for endophilin A2 highlights a unique importance of clathrin-independent intracellular trafficking in GC B-cell clonal expansion and antibody responses.doi:10.15252/embr.202051328Dessislava MalinovaLaabiah WasimRebecca NewmanAna Martínez-Riao...
(siEndoA1, siEndoA2, or siEndoA3) siRNAs.c,f–h, Immunodetection of endogenous endoA1 and endoA2 with isoform-specific antibodies, or stable low-level expression of endoA3-GFP.aContinuous uptake of anti-CD166 antibody for 10 and 30 min. Internal fluorescence quantified from confocal images...
(A) Silver stain of co-immunoprecipitations from rat brain using control mouse IgG (left lane) or anti-CPG2 monoclonal antibody (right lane). Note CPG2 at ∼105 kD and the additional band at ∼56 kD (arrow). This band was excised and identified as EndoB2 using mass spectrometry. ...
8A). It should be noticed that with the use of an antibody that recognizes the conserved N-terminal part of all endophilin A members, a major and a minor band having molecular weights in the range expected for EA2 were detected in cells transfected with empty plasmid (Fig. 8A, top left ...