2. Phosphofructokinase, the most important pacemaker enzyme of glycolysis, is inhibited by high levels of its own substrates (fructose 6-phosphate and ATP); this inhibition is overcome by AMP. In tissues, such as heart muscle, which use fatty acids as a major fuel, inhibition of glycolysis by...
this can result in excessive production of ROS. The accumulation of NADH, superoxide and ROS can potentially impede the activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is a fundamental component of glycolysis and is responsible for converting glyceraldehyde-3-phosphate into 1,3-bio...
Despite the importance of the foeto-maternal interface in the fetal development, studies of the porcine endometrium have focused mainly on early gestation because of the high embryonic mortality. However, some interesting recent studies have provided insight into the uteroplacental function of the pig ...
via decomposition of Amadori products via the Namiki pathway or as products of glycolysis. These dicarbonyls are reactive compounds. Accumulation of such dicarbonyls can not only result in AGE production but also in what is referred to as dicarbonyl stress (Singh et al., 2001), which can have...
Additionally, tumours relay essentially on anaerobic metabolism and demonstrate a higher rate of glucose uptake and thus glycolysis. This higher rate of glycolysis may bring about non-enzymatic glycation of proteins, prompting the development of AGEs [20]. The experimental data running from in-vitro ...
The centrality of the Warburg effect in tumor metabolism has been well acknowledged[12,13].In accordance with the Warburg effect,the accumulation of the metabolite lactic acid and the subsequent acidic TME are the results of enhanced glycolysis.The metabolic switch of accelerated glycolysis in cancer...
Background The metabolism of normal prostate relies on glycolysis, with prostate cancer having reduced glycolysis and increased aerobic metabolism. Advanced glycation end products (AGEs) accumulate in tissues as a result of age and glycolytic rate. Differential AGE levels were recently observed in ...
Slides were mounted in Prolong Gold Antifade Reagent (Cell Signaling Technology) and imaged with a confocal Nikon A1R inverted microscope with a 20X objective lens using a pinhole of 1.2 μm on all channels at a resolution of 1024 × 1024. In vitro glycolysis and mitochondrial ...
matrix components (collagen alpha-1[IV] chain, collagen alpha-2[VI] chain, and collagen alpha-3[VI] chain), those involved in cytoskeleton organisation (vimentin, actin, filamin-A, and talin-1), and regulators of glycolysis (fructose-bisphosphate aldolase A/B and triosephosphate isomerase, ...
In DKD, there is a shift from oxidative phosphorylation69,70 to glycolysis71 via the Crabtree72 or the Warburg69 effect. An upregulation in “mitochondrial pathways” observed in the gene set enrichment analysis in our study is likely to reflect a shift in metabolism in the kidney in diabetes...