To determine the contribution of BRCA1-mediated HR on genome stability and the suppression of mutagenesis, we clonally propagatedbrc-1mutant animals while monitoring the number of generations. In addition tobrc-1mutant animals, we also propagated null mutants for BRC-1’s binding partner BARD1/BRD...
Moreover a semiquantitative PCR of 15 cycles showed reduced rejoined products when the rejoining reaction was performed with siTRIP13- versus NT extracts from three SCCHN cell lines (Supplementary Fig. 7D). Together, these findings show that TRIP13 is involved in the NHEJ pathway. HR pathway ...
How a Spark of Innovation in Fayetteville Changed the RFID and Self-Check Paradigm: By Pushing for a Simpler, Smaller, Less Expensive RFID Self-Check Solution, We Have Begun to Change the Current Paradigm for How Those Products Are Creat... We certainly didn't set out to create a whole ...
23 P21 cDNA was PCR amplified and digested with ApaI and PstI. The digested products (225 and 200 bp) with nonhomologous ends were incubated in NHEJ buffer for 30 min. Nuclear extract of our ESCC cells was then added to the mixed buffer and further incubated for another 30 min, ...
An NHEJ assay was performed as described earlier.23P21 cDNA was PCR amplified and digested with ApaI and PstI. The digested products (225 and 200 bp) with nonhomologous ends were incubated in NHEJ buffer for 30 min. Nuclear extract of our ESCC cells was then added to the mixed buffer...