The identification of antibodies to extractable nuclear antigens (ENA) is important in the diagnosis of connective tissue diseases. Traditional gel based methods of detection such as counterimmu-noelectrophoresis (CIEP) have been replaced in recent years by more automated semi-quantitative systems such...
This distribution is highly reminiscent of the localization pattern of Ena/VASP family proteins as exemplified by labelling with polyclonal anti-VASP antibodies (pcVASP). Scale bars equal 20 μm. (c) Domain overview of the newly identified PREL1 as compared to the C. elegans MIG-10 protein, ...
In addition, the Payena dasyphylla EA fraction showed strong antioxidant activity with IC50 value of 11.64 ± 1.69 μg/mL. Conclusion These findings have shown that Payena dasyphylla might contained potential phenolic compounds that inhibiting the key enzyme in osteoarthritis development, ...
Indirect immunofluorescence assay using Hep-2 cell was performed to screen the antinuclear antibody (ANA). Euroimmun line immunoassay (LIA) and Phadia EliA ENA assay were tested to identify the antibodies against extractable nuclear antigens (ENAs). Kappa statistics was used to evaluate the degree ...
The measurement of antinuclear antibodies (ANA) is used in the autoimmune laboratory for the screening of connective tissue diseases (CTD). ANA measurements are mainly performed by indirect immunofluorescence (IIF) on HEp-2 cells or by enzyme immunoassay (EIA). The objective of this study was to...