Protein mixtures were separated by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and analyzed by immunoblotting with anti-VASP or anti-Mena antibodies. In experiments designed to determine the ability of zyxin mutants to effect the cytoskeletal changes previously shown to be ...
Next, the membrane was incubated with a blocking solution PBST containing 5% serum for 1 hour at 37°C, and then probed with goat anti-human polyclonal antibodies against MMP-3 (1:1000) (Abcam, USA) and MMP-13 (1:1000) (Santa Cruz, USA) separately overnight. Thereafter, membrane was ...