Cells (500/well) were seeded into 6-well ultra-low attachment plates (Corning, Inc., Corning, NY, USA), and cultured in suspension in DMEM/F12 (Gibco, Grand Island, NY, USA) supplemented with 2% B27, 10 ng/ml EGF and 10 ng/ml basic FGF (Gibco, MD, USA). After 7 days of ...
The homeobox factors ZEB1 and ZEB2 are bound by the zinc finger E-box and the basic helix-loop-helix factors TWIST1 and TWIST2 [91]. The loss of cadherin-1 (E-cadherin) and the acquisition of cadherin-2 (N-cadherin), together with the phenotypic change to a mesenchymal phenotype and ...
One of the basic mechanisms implicated in the pathogenesis of the toxic effect of CIS on kidney is oxidative stress [62]. Thus, in the kidney homogenates, antioxidant indicators, including GSH and SOD, as well as the marker for lipid peroxidation, MDA, were evaluated. In this study, CIS ...
(c, d). CD44 reporter plasmid was constructed by adding the CD44 promoter sequence (3135 bp) into a pGL3-basic luciferase reporter vector. MCF-7 cells were transfected with the pGL3-basic control plasmid, the CD44 reporter plasmid, the Renilla luciferase expression construct, siNC, or si...