1)向每管清洗过的CaptureBeads管中加入清洗过的EnrichmentBeads40μL。 2)室温下在混合器上旋转5min。 3)将各管放至磁力架上,等待3-5min,使磁珠吸附到磁力架上。 4)颠倒磁力架几次。 5)用1000μL吸头小心地吸除上清,不要干扰到磁珠。 6)按以下方法用EnhancingFluidXT清洗磁珠,直到上清中无可见珠子: A....
Following enrichment on the REM e System, the beadswere counted on a Beckman Coulter Counter Multisizer 3 by the manufacturer's recommended protocol. Enrichment between 5 - 20% was achieved with both samples, indicatingLiquid Handler