During this process, each unique DNA molecule in the library is bound to the surface of a bead or a flow-cell and PCR amplified to create a set of identical clones. In the case of Ion Torrent technology, a process called “templating” is used to add library mole...
A heat pipe, on the other hand, is a thermal conductor that transfers heat over a distance using a phase-change process. It contains a small amount of liquid that evaporates when exposed to heat, carrying the thermal energy to a cooler area where it condenses. The liquid then returns to...
Complete set of optimized reagents to carry out a successful DNA library preparation. Assay Time2 hours 30 minutes AMPINEXT™ DNA Size Selection Kit ENZ-GEN506 A complete set of optimized reagents for quick removal of DNA fragments of <150 bps for library preparation in next generation sequenci...
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PCR is a very complex process to mimic. Too much DNA and the 'washing machine' is too full resulting in poor amplification. What steps can we take to ensure we don't get too much DNA into the reaction (or in other words, how do w...
and indexing PCR to amplify the sample and incorporate index barcodes. The final product is a cDNA library. It is important to consider the RNA of interest and the desired types of analyses when selecting a library preparation method, as the specific mechanisms of each can make it suitable fo...
After DNA is copied, what happens to the new strands? What are some causes of DNA damage? What effect does DNA damage have? 1) Why is the sample heated to almost the boiling point in PCR? a. the DNA strands pair up b. primers attach to DNA c. the DNA denatures, or se...
The need for a One Health approach that integrates wildlife, human, and domestic animal health into a single framework is now widely recognised [1] and has gained further attention due to the recent SARS-CoV-2 pandemic [2]. In addition to their relevance as a source of pathogens that may...
First, we did identify only one Brucella spp., as our centre is in a region with a low incidence of Brucellosis. Although Brucella spp. may require prolonged incubation of blood culture bottles, published data appear to be contradictory [10]. Second, the study was conducted using BacT/Alert...
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