Embryonic stem cells(ESCs) are pluripotent cells with the ability to differentiate into any mature cell types of the trilaminargerm lines.ESCsare obtained from theinner cell massof the early (5–7 days post-fertilization) pre-implantationblastocyst. They were initially derived frommouse embryosin t...
Pluripotency refers to the ability of a cell to give rise to cells that originate from all three germ layers. Among the available human pluripotent cells, human embryonic stem cells (hESCs) are considered to have the greatest probability for practical clinical application because of their simple pr...
Roche S, Delorme B, Oostendorp R, Barbet R, Caton D, Noel D, et al., Comparative proteomic analysis of human mesenchymal and embryonic stem cells: towards the definition of a mesenchymal stem cell proteomic signature. Proteomics 2009;9(2):223-32....
This capacity is classically tested in two ways: first, the cells should yield teratomas when implanted into immunoincompetent mice (teratomas, by definition, contain representatives of all three germ layers); second, they should be able to yield cell types of these same germ layers w...
Definition of Nanog responsive genes Upon Dox induction ofNanogin our SunTag cells we observed a 2-fold increase of Nanog binding to a panel of regulatory elements displaying a wide range of enrichment levels (Fig.2a). This suggests that Dox induction may lead to functional consequences. However...
Human pluripotent stem cells (hPSCs)-derived cardiovascular progenitor cells (CVPCs) are a promising source for myocardial repair, while the mechanisms remain largely unknown. Extracellular vesicles (EVs) are known to mediate cell–cell communication, ho
Embryo-derived stem cells: Of mice and men 2001, Annual Review of Cell and Developmental Biology Oct-4: Gatekeeper in the beginnings of mammalian development 2001, Stem Cells Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells 2000, Nature Genet...
Embryonic stem cells Embryonic stem (ES) cells, first isolated from theinner cell massof blastocyst-stagepreimplantationmouse embryos,87,88are pluripotent and, thus, endowed with the ability to differentiate into all 3germ layers,mesoderm,ectoderm, andendoderm, and importantly, are able to be mainta...
Extracted splicing sequences are referred to by MaxEntScan definition. d RT-PCR analysis of splicing in the presence of Noggin RNA with or without Strap antisense MO in Xenopus early tailbud stage. Quantifications of PSI values are shown below the gel. e Fraction of mis-splicing calculated ...
The invention provides biomarker profiles of cellular metabolites and methods for screening chemical compounds including pharmaceutical agents, lead and candidate drug compounds and other chemicals using human embryonic stem cells (hESC) or lineage-specific cells produced therefrom. The inventive methods are...