Omnitor 蒸发光散射检测器(Evaporative Light Scattering Detector)作为一种通用型质量检测器,可检测挥发性低于流动相的任何样品,而无需发色基团。ELSD灵敏度比示差折光检测器高,对温度变化不敏感,基线稳定,已被广泛应用于中药和中成药组分、脂肪酸、碳水化合物、氨基酸、药物以及聚合物等的检测,尤其是用于以及其他紫外...
METHODS :HPLC-ELSD was adopted. The determine was performed on XBridge Amide column with mobile phase consisted of acetonitrile-water (75∶25,V/V)at a flow rate of 0.5 mL/min. The column temperature was set at 30 ℃,and the sample size was 10 L. The detector was evaporative light ...
ELSD detector(Alltech) 3) HPLC condition Column: Polaris C18-A 5 mm 4.6´250mm Mobile Phase: A:0.1% TFA of Water B: 0.1% TFA of Acetonitrile Flow Rate: 0.5ml/min Injection: 10μl Drift tube temperature :95℃ Gas flow:2.5L/min Impactor: Off Gain:2 Gradient: Time %A %B 0.01 ...
Method: HPLC-ELSD was carried out on Dikma Diamonsil C18(4.6 mm×250mm, 5μm)with methanol-water (99∶1) as mobile phase, the flow rate was 1.0 mL·min-1, and the detector was evaporative light-scattering detector(ELSD) , the column temperature was set at 35 ℃. Result: The ...
evaporation light scattering detector (ELSD飲食攝取之油脂在人體生理上有許多功能,其脂肪酸多寡與組成之改變,會影響細胞膜及細胞核上的訊息傳遞路徑,進而去控制特定轉錄因子的活性表現.因此,飲食脂肪與許多慢性疾病及免疫反應失常有關.飲食中脂質約95%為三酸甘油酯(Triacylglycerol, TG),其它為磷脂質,膽固醇等.而三...
Omnitor 蒸发光散射检测器(Evaporative Light Scattering Detector)作为一种通用型质量检测器,可检测挥发性低于流动相的任何样品,而无需发色基团。ELSD灵敏度比示差折光检测器高,对温度变化不敏感,基线稳定,已被广泛应用于中药和中成药组分、脂肪酸、碳水化合物、氨基酸、药物以及聚合物等的检测,尤其是用于以及其他紫外...
蒸发光散射检测器(Evaporative Light-scattering Detector)是通用型质量检测器,理论上所有的物质都能用ELSD检测出来,但对色谱柱、流动相、化合物本身等有一定的要求。 ELSD的基本原理可概括为3个过程: 1、雾化:色谱柱流出液在载气的作用下在雾化室内转变成细小的液滴(气溶胶)。
蒸发光散射检测器(EvaporativeLight-scatteringDetector)是通用型检测器,可以检测没有紫外吸收的有机物质,如人参皂苷、黄芪甲苷等。 ELSD的通用检测方法消除了常见于传统HPLC检测方法中的难点,不同于紫外和荧光检测器,ELSD的响应不依赖于样品的光学特性,任何挥发性低于流动相的样品均能被检测,不受其官能团的影响。ELSD...
HPLC evaporative light scattering detectors are used to detect and quantify compounds that lack suitable chromophores or otherwise have low UV absorbance. To achieve this analysis, an HPLC ELSD detector converts the sample eluting from an HPLC column into a fine aerosol using a nebuli...
蒸发光散射检测器(EvaporativeLight-scatteringDetector)是通用型检测器,可以检测没有紫外吸收的有机物质,如人参皂苷、黄芪甲苷等。 ELSD的通用检测方法消除了常见于传统HPLC检测方法中的难点,不同于紫外和荧光检测器,ELSD的响应不依赖于样品的光学特性,任何挥发性低于流动相的样品均能被检测,不受其官能团的影响。ELSD的...