Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromoleeules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required...
Cryo-electron tomography requires thin samples. Few cell-thinning techniques have been standardized. Here, the authors provide and validate a method to prepare, label, and image proteins at mammalian cell plasma membranes for sub-nanometer structural analysis. ...
Cryo-electron tomography (CryoET) is used to resolve biomolecules within their cellular environment down to an unprecedented resolution below one nanometer.
Discover cryo-electron tomography which delivers both structural informational about individual proteins as well as their spatial arrangement within the cell. Learn more Cryo-ET explores the ultrastructure of cells in nanometer resolution. See the data that has been obtained using this technique. ...
cryo-electron tomography 冷冻电镜 | cryo-ET工作流程中的冰污染问题及其解决方法 冷冻电子断层扫描(cryo-ET)是一种非常强大的技术,可以在接近原生状态下对细胞结构进行高分辨率的研究。由于厚于600纳米的生物样品不对电子透明,因此需要减薄才能用于冷冻电子断层扫描。为了使样品变薄,使用带有聚焦离子束和冷冻载物台...
Cryo-electron tomography: structural biology on a proteomic scale The interior of cells can be mysterious. Join other scientists as they access the inner workings of cells in 3D and at unprecedented nanoscale resolution using cryo-electron tomog...
Cryo electron tomography (cryo ET) provides 3D snapshots of the cellular context at nanometer resolution. Explore resources and solutions to implement cryo tomography into your research.
01 冷冻电镜单颗粒分析技术（cryo-EM SPA）是一种以单颗粒形式分析生物分子组装的新方法，通过将负染...
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromoleeules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required...
SARS-CoV-2 is a lipid-enveloped Betacoronavirus and cause of the Covid-19 pandemic. To study the three-dimensional architecture of the virus, we perform electron cryotomography (cryo-ET) on SARS-Cov-2 virions and three variants revealing particles of reg