cryo-electron tomography 冷冻电镜 | cryo-ET工作流程中的冰污染问题及其解决方法 冷冻电子断层扫描(cryo-ET)是一种非常强大的技术,可以在接近原生状态下对细胞结构进行高分辨率的研究。由于厚于600纳米的生物样品不对电子透明,因此需要减薄才能用于冷冻电子断层扫描。为了使样品变薄,使用带有聚焦离子束和冷冻载物台...
与日渐成熟的冷冻电镜单颗粒技术一道，冷冻电镜断层成像技术cryo-ET（cryo-electron tomography）和子断层图像平均法STA（sub-tomogram average）也正在悄然崛起，将结构生物学引导向解析柔性超大分子复合物结构、细胞原位蛋白结构等方向，应用范围覆盖蛋白复合物、病毒、细菌、细胞甚至组织，分辨率最高已达3Å [2]，是名...
Cryo-electron tomography (CryoET) is used to resolve biomolecules within their cellular environment down to an unprecedented resolution below one nanometer.
ato some watch that works the cronograf to sell 到运转cronograf卖的一些手表[translate] aCryo-electron tomography Cryo电子X线体层照相术[translate]
02 冷冻电子断层扫描（CryoET）是一种无标记的冷冻成像技术，能以纳米分辨率提供细胞环境的 3D 快照。用...
网络低温电子断层扫描技术;冷冻断层扫描 网络释义
Cryo-electron tomography requires thin samples. Few cell-thinning techniques have been standardized. Here, the authors provide and validate a method to prepare, label, and image proteins at mammalian cell plasma membranes for sub-nanometer structural analysis. ...
Cryo-Electron Tomography of Infected Cells Using in-situ cryo-ET to image 200-nanometer thin layers of virus-infected cells, the researchers discovered how the viral RNA creates the necessary factors for its replication, allowing it to quickly reproduce and possibly even protect itself against immune...
我对Cryo-ET这个技术很有兴趣,有没有什么推荐的学习材料? 答:Cryo-ET技术本身包括样品制备,电镜操作和后期的数据处理。最好是能从一个比较好的生物学问题开始入手。关于理论方面可以参考一下Joachim Frank的《Electron tomography: methods for three-dimensional visualization of structures in the cell》和《Methods ...