A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, ...
In vivo animal experiments confirmed that METTL3 upregulated EGR1 expression through IGF2BP2 and suppressed activation of the janus kinase 2 (JAK2) /signal transducer and activator of transcription 3 (STAT3) pathway, thereby inhibiting mitophagy, disrupting mitochondrial dynamics, promoting myocardial ...
7.Ogawa H, Tamaki H, Ikegame K, Soma T, Kawakami M, Tsuboi A, Kim EH, Hosen N, Murakami M, Fujioka T. The usefulness of monitoring WT1 gene transcripts for the prediction and management of relapse following allogeneic stem cell transplantation in acute type leukemia.Blood. 2003;101:1698...
Statistical significance was calculated using Two-tailed unpaired t-test in (b_left, only for Egr1, g), and Two-way ANOVA with Tukey’s multiple comparisons test in (b_p53, p21 and p16, d, f). The threshold for statistical significance was considered as *p < 0.05, **p < ...
Blood 2014; 123: 3622–3634. 31 Ghosh D. Object-oriented transcription factors database (ooTFD). Nucleic Acids Res 2000; 28: 308–310. 32 Tschan MP, Simon HU. The role of autophagy in anticancer therapy: promises and uncertainties. J Intern Med 2010; 268: 410–418. 33 Morawietz H, ...
Chronic use of NSAIDs is associated with reduced renal medullary blood flow, potentially causing severe and irreversible damage including papilla necrosis. We have previously demonstrated that chronic hyperosmolar injury in the renal medulla induces the expression of pro-fibrotic TGF-beta1 and EGR1. ...
The mRNA levels of EGR1 (C), ADAM10 (D), ICAM-1 (E), and VCAM-1 (F) in lung tissues were determined by qRT-PCR. Blood samples were isolated 6 h after LPS injection, and serum was analyzed by ELISA for TNF-α (G), IL-6 (H), and IL-1β (I) release. Histological ...
Expression of both Egr3 and RORγt resulted in reduced binding. The simplest explanation for the ability of Egr3 to prevent RORγt from inducing a target in D9 cells is that Egr3 is able to interact with RORγt. To test for an interaction between Egr3 and RORγt, we used a myc ...
These data provide the first evidence that EC-SOD activity is disrupted in chronic hypoxia, and increased EC-SOD activity can attenuate chronic hypoxic PH by limiting the hypoxic upregulation of redox-sensitive genes. 展开 关键词: Animals Mice, Inbred C57BL Humans Mice Lung Blood Vessels ...
SAH results in a typical neuronal apoptosis, concomitant with a transcriptional upregulation of Bim, E2F1 and Egr-1 but a downregulation of Bcl-2. (a) Representative pictures of the brains are shown after surgery. In SAH rat, significant blood clots mainly distribute at the base of Willis cir...