The Human EGF R (Luc) HEK293 Reporter Cell was engineered to not only express STAT3 signaling response element, but also express the receptor full length human EGF R (Gene ID: 1956). When stimulated with human EGF protein, the EGF/EGF R interaction drives STAT3-mediated luminescence. Inhibiti...
085,664), where ZNF516 peak was located in ChIP-seq assays, and constructed a luciferase reporter to test whether ZNF516 is capable of repressing theEGFRpromoter-driven luciferase reporter (EGFR-Luc). In these experiments, MCF-7 cells were co-transfected withEGFR-Luc and ZNF516 expression plas...
英文名称:EGFR (Luc) HEK293 Reporter Cell 英文同义词:EGFR (Luc) HEK293 Reporter Cell CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览EGFR (Luc) HEK293 Reporter Cell国外供应商中国供应商 EGFR (Luc) HEK293 Reporter Cell推荐供应商 ...
d Relative GREM1 promoter activity measured with different GREM1-luciferase (luc) constructs. HEK293 cells were transfected with pGL3-GREM1-luc, which includes all elements, pGL3-GREM1(b + c + d)-luc, which lacks the region ‘a’, or pGL3-GREM1(a)-luc. Luciferase activity was...
MDA-MB 468 cells were infected with lentivirus containing shRNAs against luciferase (Luc, as negative control), EGFR (shEGFR-1 and shEGFR-2), PNPase (shPNPase-2 and shPNPase-4) or c-MYC (shMYC-2 and shMYC-5) to knock down specific gene expression. The protein (B) and mRNA (C...
All proteins were expressed in HEK293F cells and purified via protein A chromatography. Non-R., non-reduced; Red., reduced. (B) SDS-PAGE analysis of the EGFRvⅢ mAbs. The scFv of each mAb was fused with 6x His-FLAG-hFc tag for purification and detection purposes. Two micrograms of ...
hek293t细胞用突变egfrpmscvpuroluc载体和pecopak病毒包装载体共转染。含病毒载体的上清随后用于感染il-3依赖性baf3细胞。移出il-3并加入嘌呤霉素使得最终选择合并的突变egfr-表达baf3克隆。3.2egfr靶向调节试验为评估化合物对egfr靶向调节的功效,在用化合物处理的nih-3t3ex20_d770_n771insnpg细胞中测量磷酸化egfr。
luc2细胞(perkin elmer 125058)的egf诱发的增殖。 40.如本文所使用的术语her2意指在人类中由erbb ‑ 2基因所编码的那个蛋白质。有关该基因或蛋白质的替代名称包括:cd340;erbb ‑ 2;her ‑ 2/neu;mln 19;neu;ngl;tkr1。该erbb ‑ 2基因经常被称为her2(源自于人类表皮生长因子受体2)。当在本文提到...
Mutation of T678, but not S1046/1047, to Ala abolished EGFR-enhanced SGLT1 expression, and this effect was reversed by treatment with proteasome inhibitor MG132 in HEK-293T cells (Fig. 7d). Mutation of T678A also reduced the interaction between EGFR and SGLT1 (Fig. 7e). These results...
aBALB/c nude mouse lungs were orthotopically transplanted with Luc-H1975 or Luc-HCC827 cells and were given Vehicle or Osi and follow-up using bioluminescence imaging (BLI) (n = 5 mice each group).b, cQuantification of the relative fluorescence activity of the region of interest (ROI...