中文名称:EGFR报告细胞系英文名称:EGFR Reporter Cell 品牌:百普赛斯产地:北京 保存条件:Frozen in liquid nitrogen纯度规格:99.9% 产品类别:细胞株/细胞系 别名:EGFR Reporter Cell货号:CHEK-ATF049 用途范围:生物是否进口:否 EGFR (Luc) HEK293 Reporter Cell ...
英文名称:EGFR (Luc) HEK293 Reporter Cell 英文同义词:EGFR (Luc) HEK293 Reporter Cell CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览EGFR (Luc) HEK293 Reporter Cell国外供应商中国供应商 EGFR (Luc) HEK293 Reporter Cell推荐供应商 ...
Osimertinib (Osi) is a widely used epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI). However, the emergence of resistance is inevitable, partly due to the gradual evolution of adaptive resistant cells during initial treatment. Here,
HEK293 cells were transfected with pGL3-GREM1-luc, which includes all elements, pGL3-GREM1(b + c + d)-luc, which lacks the region ‘a’, or pGL3-GREM1(a)-luc. Luciferase activity was measured and normalised by Renilla activity. pGL3-luciferase construct (pGL3-luc) was used ...
family)的成员的原型细胞表面受体(prototype cell ‑ surface receptor)。该家族目前具有4个密切相关的受体酪氨酸激酶:egfr、her2(erbb ‑ 2/c ‑ neu)、her3(erbb ‑ 3)和her4(erbb ‑ 4)。 3.egfr存在于细胞表面上并且通过它的特异性配体的结合而被活化,该特异性配体包括表皮生长因子与转形生长因子...
All proteins were expressed in HEK293F cells and purified via protein A chromatography. Non-R., non-reduced; Red., reduced. (B) SDS-PAGE analysis of the EGFRvⅢ mAbs. The scFv of each mAb was fused with 6x His-FLAG-hFc tag for purification and detection purposes. Two micrograms of ...
The pmirGlo luciferase expression vector (Promega) was used to construct the luciferase reporter plasmid that consists of both firefly luciferase and renilla luciferase. The 3’UTR region of EGFR mRNA was inserted after the F-luc coding sequence. The mutant EGFR luciferase reporter plasmid was ...
cell nucleus.Scale bar20 μm.cThe schematic diagram showing the Gal4-luciferase reporters.dTranscription repression by ZNF516. HEK293T or MCF-7 cells were transfected with different amounts of Gal4-ZNF516 expression plasmids, together with the indicated Gal4-luciferase reporter. Forty-eight ...
The viral vectors were produced in HEK293T cells as described30. For MUC1 and EGFR knockdown, the scrambled shRNA, the MUC1-specific shRNA sequences, and the EGFR-specific shRNA sequences were described previously30. For CREB and GRβ knockdown, the scrambled shRNA, the CREB-specific sh...
2.6. Cell Treatment For the detection of UBL3 and α-syn interaction, the cell culture medium (CM) of transfected HEK293 cells was changed to FBS (− Opti-MEM after being transfected with NGluc-UBL3, NGluc-UBL3∆5, and α-syn-CGluc plasmids for 12 h. Then CM and cells were co...