We conducted an exploratory analysis to inform the clinical utility of EGFR mutation testing in blood cell-free DNA using the cobas (R) EGFR Mutation Test v2.Wu, Yi-LongLee, VictorLiam, Chong-KinLu, ShunPark, KeunchilSrimuninnimit, VichienWang, JieZhou, CaicunAppius, AnitaButton, Peter...
Kameda T, Shide K, Yamaji T, Kamiunten A, Sekine M, Taniguchi Y, et al. Loss of TET2 has dual roles in murine myeloproliferative neoplasms: disease sustainer and disease accelerator. Blood. 2015;125:304–15. Article CAS PubMed Google Scholar Download references ...
Non-standard blood pressure (BP) is a factor in 15% to 20% of dementia cases encountered by the age of 80. Dementia's connection to hypertension remained apparent until the age of seventy-five. The regulation of blood pressure from midlife to the beginning of late-life could potentially ...
The first blood sample was collected at the time of diagnosis before the start of treatment. Circulating free DNA (cfDNA) was quantified using the QX200 Droplet Digital PCR System (Bio-Rad Laboratories, Hercules, CA). For the EGFR multiplex assays, a final PCR mix volume of 20 μL was ma...
6b). Notably, nebivolol, a beta-blocker for the treatment of patients with high blood pressure or heart failure35, was identified as the best hit as evidenced by the initial bioassay (Supplementary Fig. 8d). The docking results projected that nebivolol could fit in a dumbbell-shaped cavity ...
Major exclusion criteria included confirmation of history or presence of hemoptysis or bloody sputum, any coagulation disorder, severe or uncontrolled hypertension, or tumors invading or abutting major blood vessels. Definition of concurrent mutations and pathogenic concurrent mutations Concurrent mutations wer...
The gene alterations of the three PDXs were listed in Supplementary Table 4, note that the alterations in PDX-1R and PDX-2R were numerous due to the lack of gene mutation spectrum of paired normal tissue or blood to eliminate the redundant information of gene alterations. Even so, there ...
Cells were treated with red blood cell lysis solution for 5 min at room temperature, washed with DPBS, and resuspended in DPBS to a final concentration of 106 cells/mL in a single-cell suspension. Cells were then incubated with Fc block (BioLegend) for 15 min at room temperature, moved ...
Blood. 2015;125:304–15. Article CAS PubMed Google Scholar Download references Acknowledgements We thank Translational Medicine Core Facility of Shandong University for consultation and instrument availability that supported this work. Funding This work was supported by grants from the National Key ...
et al. Development and clinical utility of a blood-based test service for the rapid identification of actionable mutations in non-small cell lung carcinoma. J. Mol. Diagn. 19, 404–416 (2017). Article CAS PubMed Google Scholar Frampton, G. M. et al. Development and validation of a ...