However, the result obtained with this method is not always satisfactory due to the interference of endogenous gene expression. To exclude the interference from endogenous gene expression, another ap 展开 关键词: cellular level, dual expression vector, gene mutation effect, gene transcription, method ...
Identification of a mutation that perturbs NF1 agene splicing using genomic DNA samples and a minigene assay Effects on splicing can be predicted from genomic DNA sequence analysis if mutations alter highly conserved canonical splicing signals. However, it is ... M,Baralle,D,... - 《Journal ...
www.nature.com/scientificreports OPEN Childhood gene-environment interactions and age-dependent effects of genetic variants received: 10 February 2016 associated with refractive error andaccepted:25April2016 Published:13May2016 myopia: The CREAM Consortium Qiao Fan1,*, Xiaobo Guo2,3,*, J. ...
β = 0.06,P = 1.71 × 10−8, and rs1452628, with posterior probabilities of inclusion in the causal variant set of 0.56 and 0.45, respectively) close to, and eQTL of,KCNK2(TREK1). This gene regulates immune-cell trafficking into the central nervous system,...
(1998). Increase in radiation-induced HPRT gene mutation frequency from nonthermal exposure to non-ionizing 60 Hz electromagnetic fields. Radiation Research, In press, 1–30. Google Scholar Walter, R.J., Shtil, A.A., Roninson, I.B. and Holian, O. (1997). 60 Hz electric fields ...
Buchner E (1991) Genes expressed in the adult brain of Drosophila and effects of their mutation on behavior: a survey of transmitter- and second messenger-related genes. J Neurogenet 7:153–192 Buchner E, Rodrigues V (1983) Auto... Stocker, Reinhard F. - 《Cell & Tissue Research》 被引...
Effects of site-directed mutagenesis in the N-terminal domain of thermolysin on its stabilization The thermolysin variant G8C/N60C/S65P in which the triple mutation in the N-terminal domain, Gly8→Cys/Asn60→Cys/Ser65→Pro, is undertaken increases stabi... Y Kawasaki,K Yasukawa,K Inouye -...
To effectively identify driver genes, we adopted a semi-local centrality measure and gene mutation effect function to assess the effect of gene mutations on changes in gene expression patterns. Firstly, we calculated the mutation score for each gene. Secondly, we identified differentially expressed ge...
by perturbing the gene in suitable cellular or animal models. However, eQTL signals are often tissue specific8: consequently, the power to detect mechanistically informative expression effects is dependent on assaying expression data from sufficient numbers of samples across the range of disease-relevant...
With the rfaC gene supplied in trans, both the decreased expression and activity of Hly were restored to wild-type levels. Hly from the rfaC mutant strain exhibited much slower kinetics of hemolysis, a more rapid rate of decay of activity, and greater formation of apparently inactive HlyA-...