The material was surface sterilised using a 1:50 dilution of Clorox solution and thereafter rinsed using sterile water. Seeds were used to initiate in vitro callus cultures; these were grown on MS62 basal medium supplemented with BAP (benzylaminopurine, Duchefa Biochemie B.V, Haarlem, ...
Ministry of Agriculture and Land Reclamation, Egypt. Seeds were immersed in 70% ethanol for 3 min and then rinsed three times in sterile distilled water. Next, the seeds were sterilized for 30 min in 20% commercial Clorox (5% NaOCl) containing 0.5% Tween 20. After rinsing three ...