类型：慢病毒基因过表达载体 基因启动子：EF1a promoter 荧光标签：EGFP 真核细胞筛选抗性：Puromycin 原核抗性：Ampicillin pLV 〧1a 〨FP(2A)Puro 载体是基于HIV1的慢病毒基因过表达载体，采用EF1a 启动子启动目的基因的表达。EF1a 启动子来源于EF1a 启动子来自人类靶向延长因子 1α（EF1A ）基因，可在多数种类...
类型：慢病毒基因过表达载体 基因启动子：EF1a promoter 荧光标签：EGFP 真核细胞筛选抗性：Puromycin 原核抗性：Ampicillin pLV 〧1a 〨FP(2A)Puro 载体是基于HIV1的慢病毒基因过表达载体，采用EF1a 启动子启动目的基因的表达。EF1a 启动子来源于EF1a 启动子来自人类靶向延长因子 1α（EF1A ）基因，可在多数种类...
This AAV expresses DIO-mCherry driven by an ubiquitous EF1a promoter. The 1.2 Kb EF1α promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1α). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished ...
【目的】通过选用哺乳动物细胞特异性启动子—人类延伸因子1α启动子(Elonggation factor 1αpromoter,EF1-α)、利用病毒假型化工具—截断型水疱型口膜炎病毒G蛋白(Truncated Vessicular stomatitis virus protein G,VSV-GED)、添加转录后调控元件(Woodchuck hepatitis virus post-transcriptional regulatory element,WPRE)...
基因启动子:EF1α promoter 标签:Firefly Luciferase (萤火虫荧光素酶) 真核细胞筛选抗性:Puromycin 原核抗性:Ampicillin pLV-EF1α-Luci-N载体是基于HIV1的慢病毒基因过表达载体。该载体的多克隆位点位于Luciferase基因上游,在EF1α启动子驱动下表达目的基因和Luciferase的融合蛋白。EF1a启动子来自人类靶向延长因子 1α(...
SOYBEAN EF1A PROMOTER AND ITS USE IN CONSTITUTIVE EXPRESSION OF TRANSGENIC GENES IN PLANTS 发明人: LI ZHONGSEN 申请人: 申请日期: 2014-03-13 申请公布日期: 2014-09-18 代理机构: 代理人: 地址: Wilmington DE US 摘要: The promoter of a soybean translation elongation factor EF1 alpha and frag...
(MCS) and Puror. The IRES allows a protein of interest and puromycin resistance to be translated from a single bicistronic mRNA. Stable, constitutive expression of the bicistronic transcript is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after vector ...
基因启动子:EF1a promoter荧光标签:无 真核细胞筛选抗性:Hygromycin原核抗性:Ampicillin pLV-EF1a-Hygro载体是基于HIV1的慢病毒基因过表达载体,可以同时表达外源插入基因片段和Hygromycin抗性基因。外源基因插入载体中的MCS后,由EF1a启动子启动外源基因片段表达。EF1a启动子来源于EF1a启动子来自人类靶向延长因子 1α(EF1A)...
(MCS) and the mCherry gene. The IRES allows a protein of interest and mCherry to be translated from a single bicistronic mRNA. Stable, constitutive expression of the bicistronic transcript is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after vector...
pAAV-EF1a-DIO-hM3D(Gq)-mCherry(Plasmid #50460)化学遗传激活 PURPOSE Double floxed Gq-coupled hM3DDREADDfused with mCherry under the control of EF1a promoter hM3D(Gq)-mCherry Species H. sapiens (human) Insert Size (bp) 2508 Mutation ...