The basic structural/functional unit of EF-hand proteins is a pair of EF-hand motifs [34] that together form a stable four-helix bundle domain (Fig. 1) [48,61]. The pairing of EF-hands enables cooperativity in the binding of Ca2+ ions, which is essential for generating a clean respons...
A test of the acid pair hypothesis. Journal of Molecular Biology,1990,265(11):5971-5976. [148] [15] Shaw GS,Hodges RS, Sykes BD. Calcium-induced peptide association to form an intact protein domain: 1H NMR structural evidence. Science,1990,249(4966):280-283. [149] [16] Linse S,...
NMDA-myosin RLC interactions were maintained in the absence of the fourth EF-hand domain and did not require the addition of magnesium. We report that sequence similarity in the "GxxxR" portion of the incomplete IQ2 motif found in nonmuscle myosin II heavy chain isoforms likely contributes to...
SODIUM-CHANNEL NA(V)1.5FACTOR HOMOLOGOUS FACTORSOLUTION NMR STRUCTUREC-TERMINAL DOMAINX-RAY STRUCTURESEF-HAND DOMAINAPO-CALMODULIN SB Gabelli,A Boto,VH Kuhns,... - 《Nature Communications》 被引量: 19发表: 2014年 The Solution Structure of the C-terminal Modular Pair from Clostridium perfringens ...
In this report, we utilized a protein isolation strategy to enrich for factors that are associated with the cytoplasmic domain of CDHR2. In doing so, we have identified CALML4 as a novel IMAC component. In addition to the fact that CALML4 was discovered using this protocol, the evidence tha...
[42]. No other AtPLC has an EF-hand domain but the amino-terminal sequences of several other family members have two sets of a helices that may correspond to EF-hand domains [29]. The putative EF-hand loop of AtPLC1 lies between two of the a helices. The actin-binding activity of...
LETM1 contains an N-terminal domain (NTD) and a C-terminal domain (CTD) that are connected by one transmembrane helix. The CTD contains two EF-hand Ca2+ binding motifs. LETM1 was first reported as a K+/H+ exchanger22. However, it has been argued that LETM1 cannot act as a ...
The location of an alternate sequence in a constitutive region of domain IV is highlighted in dark, thick line (4). The location of the EF-hand with Y1501 an alternate isstalarbt eslietedpwroitdhuaciYn.gTshuebcuyntoitssowlicithCadVisβtisnucbtuNn-ittehramsiantil(e6a)s.tTtwheo potential...
hand motifs: the N domain (residues 1–94, a helices H1–H5) consisting of EF1 and EF2, and the C domain (residues 96– 190, a helices H6–H9), consisting of EF3 and EF4 (Figure 1A). The two domains are separated by a short loop containing a ...
E.g. the ordered pair <Brown, Bush> does not satisfy the predicate: ‘x is more powerful than y’. Describing relations: (a) A relation is expressed by a predicate. The predicate is satisfied by the set of n-tuples that define the relation. (b) We define a relation in a domain ...