NLR受体，作为植物免疫反应的核心，通过与EDS1、PAD4以及SAG101组成的信号网络协作，对病原微生物的识别和非生物胁迫做出反应。NLR蛋白通常由三个或多个结构域组成，其中NB-ARC结构域是其关键组分。NLRs可以分为三个进化枝：CNL、TNL和RNL，分别具有不同的N端结构域。NLR不仅参与病原体相关分子模式的识...
种子植物的EDS1蛋白家族成员包括EDS1、SAG101和PAD4,EDS1调控植物激素和NLR蛋白介导的免疫信号,对植物抵抗活体营养型病原菌尤其重要。EDS1与SAG101或PAD4形成异源二聚体,介导免疫信号传导。拟南芥和烟草中EDS1基因的进化分析与功能研究发现,EDS1-SAG101异源二聚体和含有CC结构域HET-S以及LOP-B结构域的helper NLR...
SAG101ETIimmunityplantSUMMARY Arabidopsis pathogen effector‐triggered immunity (ETI) is controlled by a family of three lipase‐like proteins (EDS1, PAD4, and SAG101) and two subfamilies of HET‐S/LOB‐B (HeLo)‐domain "helper" nucleotide‐binding/leucine‐rich repeats (ADR1s and NRG1s). ...
EDS1(enhanced disease susceptibility 1)是一种重要的防卫基因,其编码的信号蛋白质位于植物免疫信号网络的关键节点。EDS1的互作蛋白质包括PAD4(phytoalexin defi ciency 4)和SAG101(senescence-associated gene 101)。EDS1在以TIR-NB-LRR(toll-interleukin-1 receptornucleotide binding-leucine-rich repeat)类R蛋白(...
f, MAP kinase activation in Col-0 and eds1 pad4 sag101 treated with nlp20 or flg22 was analysed by immunoblot assay. Ponceau S-stained RUBISCO large subunit serves as a loading control. For gel source data, see Supplementary Fig. 1. Experiments in b–f were performed at least three ...
NLR可以参与植物免疫的下游关键信号传导,其中EDS1 (ENHANCED DISEASE SUSCEPTIBILITY 1) 、PAD4 (PHYTOALEXIN DEFICIENT 4 ) 和SAG101 (SENESCENCE ASSOCIATED GENE 101 ) 组成的信号网络涉及到大多数的NLRs及所有TNLs 【3】 。最近的一项研究还发现TNL激活后,EDS1,SAG101和NRG1会形成复合体并导致细胞死亡。有趣...
Biotrophic plant pathogens encounter a postinfection basal resistance layer controlled by the lipase-like protein enhanced disease susceptibility 1 (EDS1) and its sequence-related interaction partners, senescence-associated gene 101 (SAG101) and phytoalexin deficient 4 (PAD4). Maintainance of separate ...
We report the crystal structure of an Arabidopsis EDS1-SAG101 heterodimer and a derived EDS1-PAD4 heterodimer structural model. Our analysis provides a molecular basis for actions of exclusive EDS1 heterodimers with each partner in immunity. Results Distinct EDS1 Family Member Clades Are Maintained...
et al. Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity. Cell Host Microbe 14, 619–630 (2013). Article CAS PubMed Google Scholar Wong, J. E. M. M. et al. A Lotus japonicus cytoplasmic kinase connects Nod factor ...
AtEDS1-AtSAG101-AtNRG1 cell death activity is transferable to the solanaceous species, Nicotiana benthamiana, and cannot be substituted by AtEDS1-AtPAD4 with AtNRG1 or AtEDS1-AtSAG101 with endogenous NbNRG1. Analysis of EDS1-family evolutionary rate variation and heterodimer structure-guided...