When shown the structure of a hit molecule, scientists can very rapidly draw up a wish list of analogues on paper that would be worth testing against the biological target. But whether medicinal chemists have the tools to make these molecules easily is a different matter. Creating a new synthe...
The process of RNA editing can occur in various types of RNA molecules, including pre-mRNA, mature mRNA, miRNA, lncRNA, tRNA, and even viral RNA [11,12,13]. An important impact of RNA editing is its ability to challenge the central dogma of biology. When RNA editing takes place in th...
Liu and colleagues trained MoleculeSTM, a foundation model that aligns the structure and text modalities through contrastive learning, and show its utility on the downstream tasks of structure–text retrieval, text-guided editing and molecular property prediction.Liu Shengchao...
There is increasing adoption of artificial intelligence in drug discovery. However, existing studies use machine learning to mainly utilize the chemical structures of molecules but ignore the vast textual knowledge available in chemistry. Incorporating t
the specific locus targeted with sgRNA must be followed by a PAM (protospacer adjacent motif) to allow for efficient Cas9 binding [7]. In the structure of the Cas9 R-loop complex, the eleven nucleotides furthest from the PAM on the nontarget strand are disordered, suggesting they are unencumb...
Genome editing with prime editors based on CRISPR-Cas9 is limited by the large size of the system and the requirement for a G/C-rich protospacer-adjacent motif (PAM) sequence. Here, we use the smaller Cas12a protein to develop four circular RNA-mediated prime editor (CPE) systems: nickase...
2A). PE then uses the structure-specific flap endonucleases found in the cell that prefer 5′ flap as a substrate to its advantages by letting them digest the unedited 5′ flap while 3′ flap with edited DNA is ligated, forming a heteroduplex of DNA containing edited and unedited strand...
Hence, this virus can escape innate immune reaction of the host and establish a long-lasting infection in the nucleus. In human embryonic oligodendrocytes, ADAR2 KO (ADAR2-/-) enhances the BoDV-induced immune response and increases the expression of the pro-inflammatory molecules Interleukin-6 ...
Third, due to fragment deletion of the target region between the two sgRNAs, it is clear that a more complete deletion in terms of the primary structure is obtained in this manner, which is especially helpful in research on noncoding RNAs (ncRNAs) or other gene structures with biological ...
The approach essentially involves using “base editors”, which are proteins that can chemically modify the structure of a base, converting it into another base. This has numerous advantages over traditional genome editing techniques like CRISPR-Cas9. BEs are mainly used to target point mutations ...