number of nano-scaled carriers have been applied in the enzyme immobilization [4–10],and enzyme cat-alytic biotechnology is explored for its potential application.Enzyme stability is maximized with nano-scaled supports.How-ever,recovery of the nano-scaled immobilized enzymes from the reaction ...
Controlling Coupling Reaction of EDC and NHS for Preparation of Collagen Gels Using Ethanol/Water Co-Solvent. Macromol. Biosci. 2007;8:32-7.Nam K, Kimura T, Kishida A: Controlling coupling reaction of EDC and NHS for preparation of type-I collagen gels using ethanol/water co-solvents. ...
A unique approach in mechanosynthesis, joining solid-state NMR spectroscopy, X-ray crystallography, and theoretical calculations, is employed for the first time to study the mechanism of the formation of the C-N amide bond using EDC.HCl as a coupling reagent. It has been proved that EDC.HCl,...
When PGA was activated by EDC/NHS, EDC reacted with a carboxyl group in PGA and then formed an unstable amine-reactive O-acylurea intermediate, followed by a reaction between O-acylurea and NHS to yield the more stable NHS-ester. Finally, the NHS-ester was reacted with the amino group...
In the reaction mechanism, BODIPY groups are covalently linked to cholesterol molecules to form cholesterol analogues with fluorescent properties. This connection may be achieved through specific chemical reaction steps, such as esterification, acylation, or other types of chemical bonding reactions. 【中文...