Complete Western Blot Protocol Data Western Blot Western Blot with increasing amounts of CA IV protein. (top) Competitor ECL Reagent, 2 min film exposure. (bottom) Optimized ECL Reagent, 10-20 sec exposure. Primary antibody used: rabbit anti-human CA IV (1:5000); Secondary antibody used...
The Thermo Scientific Pierce Fast Western Blot Kit is a cost-effective means to obtain Western blot results quickly Researcher generated data using the Fast Western Blot Kit, ECL Substrate Comparable results obtained with classic Western blotting protocol and Fast Western Blot Kit ...
Western blot4-iodophenylboronic acidp-coumaric acid4-iodophenolWe compared several alternative ECL solutions for Western blot detection of endogenous proteins in whole cell lysates using inexpensive, commercially available reagents. Starting from an existing protocol based on p-coumaric acid (pCA) as ...
Protocol: (1)将膜浸没在缓冲液中(stripping buffer), 50-60 degree for 30 min。 (2)在 RT 中,用 TBS-T 洗脱膜 2 次,每次 10 分钟(wash the membrane for 2×10 min in TBS-T at RT) (3)封闭 (4)上一抗 (5)上二抗 在托盘中封闭(可以室温几个小时或 4 度过夜),blocking 后,用冰冷的TBST...
4.Protocol...2 5.Troubleshooting...3 6.Appendix...
Want all your Western blot buffer recipes, an ECL reagent recipe, and an easy protocol all in one place and on hand? Download our freeWestern blot cheat sheet. Originally published June 2012. Revised and updated November 2022. References ...
货号:32209,名称: Pierce ECL Western Blotting Substrate, 250 mL ,品牌:PIERCE,规格:250 ML KIT 产品介绍 订货:,, Pierce ECL Western Blotting Substrate, 250 mL介绍 Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选...
The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent si...
The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent si...
Protocol: (1)将膜浸没在缓冲液中(stripping buffer), 50-60 degree for 30 min。 (2)在 RT 中,用 TBS-T 洗脱膜 2 次,每次 10 分钟(wash the membrane for 2×10 min in TBS-T at RT) (3)封闭 (4)上一抗 (5)上二抗 第三条 在托盘中封闭(可以室温几个小时或 4 度过夜),blocking 后,用冰...