目的:探讨8-Br-cAMP对Eca-109细胞c-myc,wtp53,iNOS基因和ECFR表达的影响.方法:将贴壁培养的Eca-109细胞随机分为2组:①实验组:加8-Br-cAMP至终浓度为2×10^(-5) mol/L,培养24h;②对照组:不加8-Br-cAMP,培养24h.对2组的细胞涂片和硝酸纤维素膜(NCM)标本进行c-myc,wtp53,iNOS基因和EGFR表达检测,并...
人物简介: 一、李思容担任职务:担任东莞市朗俊五金电子有限公司监事;二、李思容的商业合作伙伴:基于公开数据展示,李思容与刘胡斌为商业合作伙伴。 财产线索 线索数量 老板履历 图文概览商业履历 任职全景图 投资、任职的关联公司 商业关系图 一图看清商业版图 合作伙伴 了解老板合作关系 ...
When getting to the camp, I got all my traps into my canoe again to have them out of sight. I put out the fire and threw the ashes around to look like an old last year's camp, and then climbed a tree. I suppose I was up for two hours; but I didn’t see or hear anything...
8-Br-cAMP目的:探讨槲皮素和8-Br-cAMP对Eca-109细胞抑癌基因表达的影响.方法:将培养的Eca-109细胞随机分成3组:①Br组,加8-Br-cAMP至终浓度为2×10-5 mol/L;②Q组,加槲皮素至终浓度为43 μmol/L;③C组,不加任何药物,只加入新培养液培养.3组细胞同时培养48 h后,分别提取mRNA.将野生型(wt)p53...
Eca-109细胞 Ca(superscript 2+)8-Br-cAMPquercetinEca-109 cell lineAim: To study the alteration of intracellular ([Ca(superscript 2+)]i) in Eca-109 cell apoptosis and Ca(superscript 2+) participation into signal transduction. Methods: The cultured Eca-109 cells were randomly divided into 3 ...
Eca-109细胞在人食管癌Eca109细胞中加以终浓度为10-5mol/L的8 BrcAMP体外培育24h后,应用酸解DNA及甲绿 派洛宁染色技术在同一标本上鉴别增殖型细胞和分化型细胞。结果:8BrcAMP处理组增殖型细胞占63%,分化型细胞占37%;而对照组增殖型细胞占83%,分化型细胞占17%。结果提示:8BrcAMP对Eca109细胞有抑制增殖和促进...
ECA-109细胞食管癌目的:比较分化诱导剂8-Br-cAMP与基因毒性药物顺铂对Eca-109细胞DNA polβ及wtp53基因表达和多药耐受蛋白的影响.方法:分别制备生物素和地高辛标记的polβ探针和生物素标记的野生型p53(wtp53)探针,并检测各探针灵敏度.将培养的人食管癌Eca-109细胞分组如下:①8-Br-cAMP组(Br组);②不加8-Br-...
8-Br-cAMP细胞凋亡食管癌Eca-109治疗DifferentiationApoptosisGene expression8-Br-cAMPEca-109 cell lineAIM: To investigate the effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109, and the related gene expression.METHODS: The cultured Eca-109 cells were ...
在人食管癌Eca-109细胞中加以终浓度为10^-5mol/L的8-Br-cAMP体外培育24h后,应用酸解DNA及甲绿-派洛宁染色技术在同一标本上鉴别增殖细胞和分化细胞。陈奎生郑州大学第一附属医院张林庆河南医科大学组织学与胚胎学教研室河南医科大学学报陈奎生;张林庆.8—Br—cAMP对人食管癌Eca—109细胞增殖与分化的效应.河南医科...
SCA. ECa. X-1 (El Campamento) borehole, drilled in the southwestern region of the Austral basin in the Santa Cruz province. Fourteen genera and nineteen species were recognized. Most of the studied forms are strongly ornamented and a few of them are smooth or delicately punctated. Of the...