Quantitative PCR (qPCR) was performed in triplicate for each data point shown using an ABI 7500, Maxima SYBR green/ROX mix (Thermo Fisher Scientific) and 1 μL of cDNA with the following primers: BRLF1 5′-AATTTACAGCCGGGAGTGTG-3′ (sense) and 5′-AGCCCGTCTTCTTACCCTGT-3′ (antisense...
A A PCR array was used to detect changes in the expression of autophagy-related genes in LMP1-positive NPC cells compared with LMP1-negative NPC cells. B Quantitative RT-PCR and C Western blots were performed to analyze LMP1 and BNIP3 expression in NPC cells. D Quantitative RT-PCR and E...
(1) Antibody-conjugated magnetic bead sorting was performed, followed by quantitative PCR or FISH [Citation5]. (2) Flow cytometry (3) Histopathology using in situ hybridization for EBV-encoded small RNA (EBER). EBER is produced by cells latently infected by the EBV virus, and EBER can be ...
Copy numbers of EBV-DNA in plasma were assessed by real-time quantitative PCR. Patients in whom disease recurrence was suspected underwent image evaluation, esp. \\{PET\\} scan, and tissue proof if it is feasible. Lesions of undermined nature were confirmed by sequential follow-up. Results ...
Quantitative PCR would help to determine the declining or rising of viral load that could be important for pre-emptive antiviral therapy. In the present study, there was no biopsy available for the CMV DNA detection to confirm the diagnosis of end organ- disease. Therefore, the clinical ...
2.6. RNA isolation and quantitative real-time PCR Total RNA was extracted from cells with TRIzol reagent (Invitrogen). cDNA was synthesized with the PrimeScript RTreagent Kit (Promega, Madison, WI, USA). For miR-204, reverse transcription and qRT-PCR reactions were performed by means of a q...
Quantitative PCR would help to determine the declining or rising of viral load that could be important for pre-emptive antiviral therapy. In the present study, there was no biopsy available for the CMV DNA detection to confirm the diagnosis of end organ-disease. Therefore, the clinical ...
We have demonstrated, using quantitative real-time PCR, the expression of BLLF3 in tumors (9/10) obtained from SCID mice injected with C666-1 cells, which is an EBV-genome positive NPC cell line [38]. Zhang et al. [39], using microarray technology, demonstrated the expression of BLLF3...