Indoor fungal contamination of moisture-damaged and allergic patient housing analysed using real-time PCR. Aims: The aim of our study was to compare, using real-time (Rt) PCR, quantitative levels of five fungal species in three kinds of dwellings. Methods and R... AP Bellanger,G Reboux,S...
Copy numbers of EBV-DNA in plasma were assessed by real-time quantitative PCR. Patients in whom disease recurrence was suspected underwent image evaluation, esp. \\{PET\\} scan, and tissue proof if it is feasible. Lesions of undermined nature were confirmed by sequential follow-up. Results ...
A A PCR array was used to detect changes in the expression of autophagy-related genes in LMP1-positive NPC cells compared with LMP1-negative NPC cells. B Quantitative RT-PCR and C Western blots were performed to analyze LMP1 and BNIP3 expression in NPC cells. D Quantitative RT-PCR and E...
Increase of p21 mRNA and decrease of c-myc expression was confirmed by semiquantitative RT-PCR and Western blotting (Fig. 6, A and B). Nonphosphorylated Rb protein was also increased by western blot in EBV-converted BL cells. Both down- regulation of c-myc expression in BL cells and p21...
2.6. RNA isolation and quantitative real-time PCR Total RNA was extracted from cells with TRIzol reagent (Invitrogen). cDNA was synthesized with the PrimeScript RTreagent Kit (Promega, Madison, WI, USA). For miR-204, reverse transcription and qRT-PCR reactions were performed by means of a q...
We have demonstrated, using quantitative real-time PCR, the expression of BLLF3 in tumors (9/10) obtained from SCID mice injected with C666-1 cells, which is an EBV-genome positive NPC cell line [38]. Zhang et al. [39], using microarray technology, demonstrated the expression of BLLF3...