EBV DNA was detected more frequently in whole blood (55%) than in plasma (18%). The detection rate of CMV DNA was similar between the two sample types. The correlations between the viral loads in the two sample types were 0.515 and 0.688 for EBV and CMV, respectively. Among paired ...
将步骤1提取的DNA、阳性质控品、阴性质控品各取4μL,加入相应的反应管中,盖好管盖,混匀,短暂离心。 PCR扩增(核酸扩增区) 4.1将待检测反应管置于荧光定量PCR仪反应槽内; 4.2设置好通道、样品信息,反应体系设置为25ul;荧光通道选择: 检测通道 (Reporter Dye)FAM, 淬灭通道(Quencher Dye)NONE,请勿选择 ROX 参比...
1)EB病毒(EBV)核酸试剂盒*卖RT-PCR可以检测组织、细胞、血液、细菌等很多材料,不同的样本有不同要求,实验前请充分沟通确认实验方案和样本情况; 2)客户尽可能提供实验的背景信息、物种、基因准确的名称和ID号; 3)客户尽量不要提供DNA或RNA样品。 4)样本保存于液氮或干冰,也可以保存于Trizol液中。
诱导化疗疗程与预后呈正比。随着诱导化疗周期增加血浆EBV-DNA转阴率逐渐增加,4个周期化疗后血浆EBV-DNA降0作为预测进展指标的敏感性和特异性均优于其他两组。 关键词: 鼻咽肿瘤/诱导化学疗法;鼻咽肿瘤/同期放化疗法;EB病毒;脱氧核糖核酸 ABSTRACT ObjectiveTo investigate the value of induction chemotherapy in the...
Use of real time PCR to measure Epstein Barr virus genomes in whole blood. J Immunol Methods. 2002;270:259–67. PubMed CAS Google Scholar Niesters HG, van Esser J, Fries E, Wolthers KC, Cornelissen J, Osterhaus AD. Development of a real-time quantitative assay for detection of Epstein...
PCR for immunoglobulin gene rearrangements DNA was extracted from whole tissue formalin-fixed paraffin-embedded tissue sections and amplified by PCR for detection of Ig (Ig heavy [IGH@] and Ig kappa locus [IGK@]) gene rearrangements, as published previously.27 For the IGH locus, we employed con...
Quantitative PCR analysis of human cells in HuCD34 engrafted mice A primer-probe mix specific for the EBV BALF5106 gene was used to quantify EBV in DNA extracted from blood or spleen in hCD34 engrafted NSG recipient mice at the time points described. Each 25 μL qPCR reaction contained 12.5...
DNA fragments bound to LEF-1 were quantified by using specific quantitative PCR primers for the CD1d promoter region (forward: CTG CTA AAG CAA GGA CTT TGA TCC; reverse: GCA GGA CAT AAG GTT GTG TCT GTG TT) and ABI 7500 Fast Real-Time PCR System (Applied Biosystems). The quantity of ...
PCR was run on an ABI Prism 7700 Sequence Detector (Applied Biosystems) and sample analyzed in duplicates. KSHV in spleen and blood DNA were determined by quantitative amplification of ORF26 as described below. The presence of KSHV DNA in blood and spleen in KSHV infected and EBV+KSHV dually...
qRT-PCR was performed and analyzed with a StepOnePlus-PCR cycler (Thermo Fisher Scientific). Cycle threshold (CT) values were used to calculate the number of copies of EBV genomes by a standard dilution curve provided with the kit and adjusted to the respective DNA concentrations. Analysis of ...