E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 ...
Of the known binding partners for E2F1, we queried increased OGA expression through the O-GlcNAc modifica- N I V whether retinoblastoma 1 (Rb1) might be involved. Rb1-defi- tion of RNA polymerase II, whereas OGT levels decreased (13). E R cient mouse embryonic fibroblasts showed ...
细胞周期相关转录因子E2F1,最早是在研究 [1] 腺病毒E2启动子的过程中被发现的,在多种生理 和病理过程中发挥重要作用,具有调控细胞周期进 展、诱导细胞凋亡的功能。E2F1基因全长约为11 000bp,定位于人20号染色体的长臂上即20q11.2, mRNA全长为2486bp,由7个外显子与6个内含 ...
Western blot analysis confirmed the loss of Rb1 expression in Rb1−/− MEFs (Fig. 7A). The qPCR analysis showed significantly elevated Ogt and Mgea5 mRNA expression in Rb1−/− cells compared with WT cells (Fig. 7B). Western blot analysis showed a significant increase in both OGT ...
E2F transcription factors are known as central regulators of cell-autonomous effects on cell cycle progression in most mammalian cells by cooperating with the Retinoblastoma (RBs: pRb/RB1, RBL1/p107 and RBL2/p130) family proteins that upon binding switch the E2F transcription factor complex from...
Moreover, using a luciferase assay we found that Chk1’s expression is controlled by p53 and RB/E2F1 at the transcriptional level. Additionally, we present preliminary data suggesting a posttranscriptional regulation mechanism, involving miR-195 and miR-503, which are inversely correlated with ...
As one of the most common primary intraocular carcinomas, retinoblastoma generally stems from the inactivation of the retinoblastoma RB1 gene in retinal ce... Y Yang,XW Peng - 《Bioscience Reports》 被引量: 0发表: 2018年 lncRNA ANRIL调控ATM-E2F1信号通路在胆囊癌上皮间质转化中的作用 目的 探讨...
ELISA结果示:在DMC组,RB1和P53蛋白表达显著增高,而Bcl-2,E2F1 和E2F3蛋白的表达显著降低.结论:DMC能诱导K562细胞发生凋亡,并能引起细胞内miRNA及相关基因的表达... 张帅,李有杰,张超,... - 《中国病理生理杂志》 被引量: 0发表: 2011年 E2F1在肿瘤中的研究进展 E2F1是E2F转录因子家族的重要一员,参与人体生命...
Loss of E2F-1 reduces tumorigenesis and extends the lifespan of Rb1(+/-)mice. Nat Genet 1998; 18: 360–364. Article CAS Google Scholar Xie Y, Wang C, Li L, Ma Y, Yin Y, Xiao Q . Overexpression of E2F-1 inhibits progression of gastric cancer in vitro. Cell Biol Int 2009; 33...
E2F1 and RB1 expression plasmids were a kind gift by Dr. Sudhakar Baluchamy, Department of Biotechnology, Pondicherry University, INDIA. The details of the primers are listed in Supplementary Table S3. To generate promoter constructs, human ESRP1 (Gene ID: 54845) promoter sequence was retrieved...