p16一Rb:-HDACl一E2F1在胆道癌细胞系中的表达 目的研究p16一RbHDACl一E2F1中各种蛋白及其mRNA在肝外胆管癌和胆囊 癌细胞系中的表达情况。 方法用免疫组织化学和RT—PCR方法检测肝外胆管癌细胞系(QBC。、KMBC、 OZcell lines)和胆囊癌细胞系(Mz—ChA一1cellline)中p16一Rb—HDACl一E2F1 ...
HDAC1及E2F1在肾透明细胞癌中的表达及意义.pdf,中文摘要 HDACl及E2F1在肾透明细胞癌中的表达及意义 摘要 目的:肾细胞癌(renalcell cellrenalcell 见的恶性肿瘤,其中60%.85%为肾透明细胞癌(clear 胞癌早期不易被发现,患者一般没有明显的临床症状,且不到1/3的患 者出
Mechanistically, HR488B obviously decreased the phosphorylation level of the retinoblastoma protein (Rb), and subsequently prevented the release of E2F1 from the E2F1/Rb/HDAC1 complex, which ultimately suppressed the growth of CRC cells. Overall, our study suggests that HR488B, a novel and ...
研究背景:人乳头瘤病毒 (HPV) 的 E7 蛋白通过诱导静止细胞进入细胞周期,在病毒基因组复制中发挥着至关重要的作用,从而促进细胞 DNA 复制机制在病毒基因组复制中的有效利用。E7 表现出转化和反式激活活性,破坏 RB1-E2F1 复合物并激活 E2F1 调节的 S 期基因。它干扰 HDAC1 和 HDAC2 介导的宿主组蛋白脱乙酰化,...
HDAC1及 E2 F1在肾透明细胞癌中的表达及意义 目的:探讨组蛋白去乙酰化酶1( HDAC1),转录因子E2 F1在人肾透明细胞癌中的表达和意义.方法应用免疫组织化学法检测51例肾透明细胞癌组织,24例癌旁正常组织中HDAC1,E2F1... 朱鑫,王亚轩,常学良,... - 《《河北医药》 2015年19期 2885-2888页 ISTIC CA》 被引量...
Therefore, the binding of the viral transforming proteins to the pRB-pocket region results in the loss of E2F and HDAC1 binding. We thus asked whether the HMGA2/pRB interaction could displace HDAC1 from the pRB/E2F complex, and so account for E2F1 activation. To this aim, we used the ...
Rb: retinoblastoma protein Cdk: cyclin-dependent kinase BrdU: bromodeoxyuridine siRNA: small interfering RNA shRNA: short hairpin RNA ChIP: chromatin immunoprecipitation HDAC: histone deacetylase HSP90: heat shock protein 90 PARP: poly (ADP-ribose) polymerase ...
In addition, E2F1 may displace HDAC1 from the C terminus of Sp1, but E2F1 itself could interact with retinoblastoma protein (RB), which again recruits HDAC1 or HDAC2 to promoter regions, thus keeping the promoter inactive39. However, previous analyses of the c-myc, dihydrofolate reductase (...
Furthermore, elevated miR-449a downregulates cell cycle regulator CDC25A and oncogene HDAC1. By targeting genes involved in controlling pRb/E2F1 activity, miR-449a regulates cell cycle progression and apoptosis and consequently enhances the radiosensitivity of PC-3 cells. Thus, miR-449a, as a ...
Control of E2F1 activity is restricted via its interactions with RB1 and HDAC1. However, the detailed regulatory mechanisms underlying the E2F1/HDAC1 complex remain elusive. Here, we report that nemo-like kinase (NLK) boosts cell cycle progression, which facilitates tumor development by releasing ...