Continuous separation of E. coli fermentation broth using a CEPA® LE laboratory centrifuge systemAguiar, Marcelo
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This suspension was transferred to a sterile 15 mL centrifuge tube and incubated at 37° C. for 3 hours to recover antibiotic resistance. After the recovery period, the suspension was plated onto, or diluted into, DM-P5 media and incubated at 37° C. to obtain transformants. 23 Liter ...
pleuropneumoniae (107 CFU/mL), and 8.9 mL TSB were added into 15 mL centrifuge tubes and cultured in an incubator (37 ℃, 5% CO2). The population of A. pleuropneumoniae was counted by the agar plate method at 0, 1, 3, 6, 9, 12, and 24 h, respectively. The detection limit of...
Centrifuge beads 1 min at 3500 rpm, room temperature.Transfer supernatant into new 1.5 ml tube. Add 80 μl TE and 1µl RNase A (20mg/ml) to the eluted DNA and to 100µl of the control DNA (see above) Incubate for 90 min at 42°C add 20 μl Proteinase K to each tube and...
coli ClpB oligomers dissociate on the time scale of minutes or shorter. In buffer H with 100 mM NaCl, the dissociation rate constant for ClpB hexamer was meas- ured to be 1.3 (1.0, 1.6) 3 1023 s21, indicating dissocia- tion is slower but still on the time scale of minutes. Going ...
addition of theE. coliO157:H7 strain. The remaining 5-day cultures were aliquoted into 50 mL centrifuge tubes, 15 mL per tube, then one sterile SS (18 × 18 × 2 mm) chip was placed in each of the tubes to be immersed in the culture as a platform on which biofilms...
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Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains opt