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5.35.3.1.2 Facilities in cell culture lab These include the following: • Dressing room: with isolation hangers on the wall. • Sterile operating area: equipped with laminar flow hoods, CO2 incubators, centrifuge, and an inverted microscope. • Sterile buffer room: provided with a refrigerat...
(relative centrifugal force, rcf). the correct rotor speed can be calculated by measuring the maximum radius of your rotor and entering the information into the table found at this website which can be reached via this link .for example, to centrifuge cells at ...
The centrifuge method allows the contents of a cylindrical galvanic cell (1) with a powdered zinc anode and a gas cathode to be centrifuged by rotation about its axis, at a speed sufficient for causing the zinc powder to form a hollow cylinder between the cathode and the outer wall of the...
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An equivalent of 0.5 ml cell culture at an OD660 of 0.5 was harvested by centrifugation in a table-top centrifuge at max speed and the pellets were snap-frozen in liquid nitrogen and stored at −20 °C. Cells were lysed in 100 µl lysis buffer (5 ml contain: 10 mM ...
Each tissue sample was transferred to a sterile 10-cm culture dish and cut into < 1 mm3segments before being transferred to a 50-ml centrifuge tube. Each tissue type was then digested in the DMEM (Sigma-Aldrich, USA) containing 100 μg/ml collagenase type I and 100 μg/ml type II (...
Note that this list is not all inclusive; the requirements for any cell culture laboratory depend the type of work conducted. Basic equipment • Cell cultu ood (i.e., laminar-flow hood or biosafety cabinet) • Incubator (humid CO2 incubator recommended) • Water bath • Centrifuge ...
Instead of plating, the cell suspension is divided into sterile, screw-cap centrifuge tubes (15-ml volume) and centrifuged at room temperature for 5 minutes at low speed. The supernatant is decanted and the cell pellet resuspended in a solution containing 90% FBS and 10% glycerol. The volume...