BACKGROUND & AIM: To detect the germline mutations in E-Cadherin gene (CDH1) and to investigate their possible role in familial gastric cancer. MATERIAL AND METHODS: With PCR, denaturing high-performance liquid chromatography (DHPLC) and direct sequencing, we screened all exons of the E-cadherin...
1)E-cadherin geneE-cadherin基因 1.PURPOSE: To construct ACC-M cell line of RNA interference-mediated knockdown of DNMT1, and to explore the effects on E-cadherin gene expression in this cell line.方法:设计靶向干扰DNMT1的shRNA序列,构建携带该序列的慢病毒载体并转导ACC-M细胞,对筛选出的抗性克隆...
1) E-cadherin gene E-cadherin基因1. PURPOSE: To construct ACC-M cell line of RNA interference-mediated knockdown of DNMT1, and to explore the effects on E-cadherin gene expression in this cell line. 方法:设计靶向干扰DNMT1的shRNA序列,构建携带该序列的慢病毒载体并转导ACC-M细胞,对筛选出的...
Jordaan G, Liao W, Sharma S (2013) E-cadherin gene re-expression in chronic lymphocytic leukemia cells by HDAC inhibitors . BMC Cancer 13 : 88.Jordaan G, Liao W, Sharma S (2013) E-cadherin gene re-expression in chronic lymphocytic leukemia cells by HDAC inhibitors. BMC Cancer 13(1):...
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO 细胞定位 Cell junction , Cell membrane 功能与背景 This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadhe...
3.左侧一列列出的突变(mutation)情况具体包括了:移码突变(Frameshift)、无义突变(Nonsense)、剪切位点突变(Splice site)、基因内缺失(Intragenic deletion)、全基因缺失(Whole gene deletion)。 不过,本文原作者经验表明,E-cadherin免疫组化检测在很多情况下...
关键词:猪E-cadherin基因基因克隆RACE Ⅱ 广西大学硕士论文猪E-cadherin基因eDNA全序列的克隆与分析及其在孤雌胚中表达的初步研究 PRELIMINARYSTUDIESONCLONINGAND SEQUENCE ANALYSIS OFCOMPLETEcDNAOFPORCINEE.CADHERINGENEAND EXPRESSIONOFE.CADHERINDURINGEARIYDEVELOPMENT OFPORCINEB▲RTHENoGENETICEMBRYOS E-cadherin(E-cad...
[目的]构建E-cadherin过表达和RNAi慢病毒载体,转染A549肿瘤细胞,观察E-cadherin的表达。[方法]根据Gene Bank E-cadherin的序列,选择设计两条序列和一条无关RNAi序列作为对照,通过Mlu I和Nde I两个酶切位点,将设计好的siRNA片段插入包装质粒PHR-SIN-CSGW-H1a(PHR)构建E-Cadherin RNAi慢病毒载体PHR-EA、PHR-EB...
[目的]构建E-cadherin过表达和RNAi慢病毒载体,转染A549肿瘤细胞,观察E-cadherin的表达。[方法]根据Gene Bank E-cadherin的序列,选择设计两条序列和一条无关RNAi序列作为对照,通过Mlu I和Nde I两个酶切位点,将设计好的siRNA片段插入包装质粒PHR-SIN-CSGW-H1a(PHR)构建E-Cadherin RNAi慢病毒载体PHR-EA、PHR-EB。
E-钙黏着蛋白(E-cadherin)是定位在细胞表面的跨膜糖蛋白,主要存在上皮组织中,可作为受体与单核细胞增生李斯特氏菌(Listeriamonocytogenes)的内化素(InlA)[8]和肺炎链球菌(S.pneumoniae)表面蛋白(PspA)[9]相连接,细菌可依靠这种连接侵入宿主细胞。目前尚未见关于尼罗罗非鱼E-钙黏着蛋白(On-ECdh)功能的相关报道。笔者...